Practical strategies for small-molecule probe development in chemical biology.
Practical strategies for small-molecule probe development in chemical biology.
复制标题
化学生物学中小分子探针开发的实用策略。
DOI：
10.1007/978-1-4939-2269-7_17
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Hong,CharlesC
中科院分区：
文献类型：
--
作者：
Hempel,JonathanE;Hong,CharlesC
有效的识别，选择和实施的小分子的生物系统的审讯需要一个复杂的理解的化学原理，其细胞活动。虽然已经发表了很多关于使用筛选技术在正向化学遗传学平台和小分子目标识别，较少的重点放在详细的战略，评估，选择和优化筛选命中。本章提供了实用的工具，用于识别和开发有前途的筛选命中化合物成为生物发现的有效工具。
The effective identification, selection, and implementation of small molecules for the interrogation of biological systems require an intricate understanding of the chemical principles underlying their cellular activities. While much has been published regarding the use of screening techniques in forward chemical genetics platforms and on small-molecule target identification, less emphasis has been placed on detailed strategies for evaluating, selecting, and optimizing screening hits. This chapter provides practical tools for identifying and developing promising screening hit compounds into effective tools for biological discovery.