A COMPARISON OF CLONOGENIC, MICROTETRAZOLIUM AND SULFORHODAMINE-B ASSAYS FOR DETERMINATION OF CISPLATIN CYTOTOXICITY IN HUMAN OVARIAN-CARCINOMA CELL-LINES
A COMPARISON OF CLONOGENIC, MICROTETRAZOLIUM AND SULFORHODAMINE-B ASSAYS FOR DETERMINATION OF CISPLATIN CYTOTOXICITY IN HUMAN OVARIAN-CARCINOMA CELL-LINES
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DOI：
10.1016/0959-8049(93)90394-u
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发表时间：
1993-01-01
影响因子：
8.4
通讯作者：
HAMILTON, TC
中科院分区：
文献类型：
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作者：
PEREZ, RP;GODWIN, AK;HAMILTON, TC
一种基于磺酰罗丹明B（SR B）对细胞蛋白定量染色的测定方法最近被NCI用于大规模新药筛选。然而，关于SRB测定法是否与其他已建立的方法相当的数据很少。用SRB法和克隆形成法测定了16株人卵巢癌细胞系中顺铂的细胞毒活性，用MTT法测定了7株人卵巢癌细胞系中顺铂的细胞毒活性。细胞系来源于未经治疗的患者（其中一些是针对体外顺铂抗性而选择的）和临床上对基于顺铂的化疗难治的患者。所有细胞系的SRB染色与细胞数呈良好的线性相关（r = 0.972-0.999）。通过SRB和克隆形成试验获得的IC 50值（r = 0.824，P = 0.000022）高度相关，尽管SRB试验中获得的值一致较高。SRB法测定的IC 50值与MTT法测定的IC 50值也具有良好的相关性（r = 0.906，P = 0.0010）。总体而言，SRB测定允许快速和可靠的评估顺铂敏感性在这些细胞系和比较有利的克隆和MTT测定。
An assay based upon quantitative staining of cellular protein by sulforhodamine B (SRB) has recently been adopted by the NCI for large-scale screening of new drugs. However, there are few data available regarding whether the SRB assay is comparable to other established methods. Cisplatin cytotoxicity was determined in 16 human ovarian carcinoma cell lines by both SRB and clonogenic assays, and by microtetrazolium (MTT) assay in seven cell lines. Cell lines were derived from untreated patients (some of which were selected for cisplatin resistance in vitro) and from patients clinically refractory to cisplatin-based chemotherapy. There was excellent linear correlation between SRB staining and cell number in all cell lines (r = 0.972-0.999). IC50 values obtained by the SRB and clonogenic assay (r = 0.824, P = 0.000022) were highly correlated, although values obtained in the SRB assay were uniformly higher. IC50 values obtained by SRB assay also correlated well with results obtained by MTT assay (r = 0.906, P = 0.0010). Overall, the SRB assay permitted rapid and reliable assessment of cisplatin sensitivity in these cell lines and compared favourably with clonogenic and MTT assays.