INVIVO P-31 NUCLEAR MAGNETIC-RESONANCE REVEALS LOWERED ATP DURING HEAT-SHOCK OF TETRAHYMENA
INVIVO P-31 NUCLEAR MAGNETIC-RESONANCE REVEALS LOWERED ATP DURING HEAT-SHOCK OF TETRAHYMENA
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DOI：
10.1126/science.6828852
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发表时间：
1983-01-01
期刊：
影响因子：
56.9
通讯作者：
SHULMAN, RG
中科院分区：
文献类型：
--
作者：
FINDLY, RC;GILLIES, RJ;SHULMAN, RG
细胞合成一组特有的蛋白质(热休克蛋白)，以响应温度的快速升高或某些其他应激处理。用P-31核磁共振波谱技术在活体内检测了温度跃升对2种藻类的影响。在不同温度下启动热休克反应的四膜虫[I.梨形和嗜热四膜虫]。当两个物种中的任何一个跳到强烈诱导热休克蛋白合成的温度时，细胞内的ATP立即下降了50%。这种新的三磷酸腺苷浓度维持在热休克温度。
Cells synthesize a characteristic set of proteins (heat shock proteins) in response to a rapid temperature jump or certain other stress treatments. The technique of P-31 NMR spectroscopy was used to examine in vivo the effects of a temperature jump on 2 spp. of Tetrahymena [I. pyriformis and T. thermophila] that initiate the heat shock response at different temperatures. An immediate 50% decrease in cellular ATP was observed when either species was jumped to a temperature that strongly induces synthesis of heat shock proteins. This new ATP concentration was maintained at the heat shock temperature.