Injectable alginate hydrogel loaded with GDNF promotes functional recovery in a hemisection model of spinal cord injury
Injectable alginate hydrogel loaded with GDNF promotes functional recovery in a hemisection model of spinal cord injury
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DOI：
10.1016/j.ijpharm.2013.07.045
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发表时间：
2013-10-15
影响因子：
5.8
通讯作者：
des Rieux, Anne
中科院分区：
文献类型：
--
作者：
Ansorena, Eduardo;De Berdt, Pauline;des Rieux, Anne
我们假设，通过可注射的海藻酸盐水凝胶原位凝胶化在脊髓病变中局部递送GDNF将支持脊髓可塑性和功能恢复。与未配制的GDNF（游离GDNF）相比，GDNF包封在微球中的水凝胶释放速度减慢。将游离gdnf水凝胶注入大鼠脊髓半切模型，观察到大鼠损伤处有更多的神经丝。当动物用装载GDNF微球的水凝胶处理时，在病变周围的组织中检测到更多的生长神经突。未处理的脊髓病变观察到强烈的GFAP（星形胶质细胞）、低β III微管蛋白（神经细胞）和RECA-1（内皮细胞）染色，而gdnf处理的脊髓病变部位GFAP染色较少，内皮细胞和神经纤维浸润较多。与GDNF微球水凝胶处理的动物和未处理的动物相比，用游离GDNF水凝胶处理的动物表现出更好的功能恢复。(C) 2013 Elsevier B.V.版权所有
We hypothesized that local delivery of GDNF in spinal cord lesion via an injectable alginate hydrogel gelifying in situ would support spinal cord plasticity and functional recovery. The GDNF release from the hydrogel was slowed by GDNF encapsulation in microspheres compared to non-formulated GDNF (free GDNF). When injected in a rat spinal cord hemisection model, more neurofilaments were observed in the lesion when the rats were treated with free GDNF-loaded hydrogels. More growing neurites were detected in the tissues surrounding the lesion when the animals were treated with GDNF microsphere-loaded hydrogels. Intense GFAP (astrocytes), low beta III tubulin (neural cells) and RECA-1 (endothelial cells) stainings were observed for non-treated lesions while GDNF-treated spinal cords presented less GFAP staining and more endothelial and nerve fiber infiltration in the lesion site. The animals treated with free GDNF-loaded hydrogel presented superior functional recovery compared with the animals treated with the GDNF microsphere-loaded hydrogels and non-treated animals. (C) 2013 Elsevier B.V. All rights reserved.