Slow synaptic potentials in neurones of the myenteric plexus.
Slow synaptic potentials in neurones of the myenteric plexus.
复制标题
肌间丛神经元的突触电位缓慢。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
R. North
中科院分区：
文献类型：
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作者：
S. Johnson;Y. Katayama;R. North
1.在体外对豚鼠回肠肌间神经丛中的神经元进行细胞内记录。在距离穿刺细胞30 - 100 μ M处记录肌间神经节表面对局灶性刺激的响应电位变化。2.包含单脉冲的刺激诱发S细胞中的胆碱能兴奋性突触后电位（“快速”e. p. s. p. s）。在一个小比例（20%）的S和AH细胞，这样的刺激也诱发去极化电位的时间过程约1000倍慢于快速epsp。由重复脉冲（高达20 Hz，持续2秒）组成的刺激在较高比例的神经元（42%）中诱发了缓慢去极化电位。这些刺激引起9%的细胞超极化电位变化，在极少数细胞中观察到双相变化。4.缓慢去极化和缓慢超极化反应在阿托品（高达5 μ M）和六甲双铵（高达200 μ M）中持续存在，但通过更换为无钙溶液可逆地消除。5.慢去极化反应是由膜钾电导失活引起的，慢超极化反应是由膜钾电导激活引起的。
1. Intracellular recordings were made in vitro from neurones in the myenteric plexus of the guinea‐pig ileum. Potential changes were recorded in response to focal stimulation of the surface of the myenteric ganglion at a distance of 30‐100 microM from the impaled cell. 2. Stimuli comprising single pulses evoked cholinergic excitatory post‐synaptic potentials ('fast' e.p.s.p.s) in S cells. In a small proportion (20%) of both S and AH cells such stimuli also evoked depolarizing potentials with a time course about 1000 times slower than that of the fast e.p.s.p. 3. Stimuli comprising repeated pulses (up to 20 Hz for 2 sec) evoked the slow depolarizing potentials in a higher proporton of neurones (42%). These stimuli caused a hyperpolarizing potential change in 9% of cells, and in a very few cells biphasic changes i membrane potential were observed. 4. Both the slow depolarizing and the slow hyperpolarizing responses persisted in atropine (up to 5 microM) and hexamethonium (up to 200 microM), but were reversibly abolished by changing to calcium‐free solutions. 5. Evidence is presented which suggests that the slow depolarizing response is caused by inactivation of the membrane potassium conductance, and the slow hyperpolarizing response is due to activaton of the potassium conductance.