Variations of human papillomavirus type 58 E6, E7, L1 genes and long control region in strains from women with cervical lesions in Liaoning province, China
Variations of human papillomavirus type 58 E6, E7, L1 genes and long control region in strains from women with cervical lesions in Liaoning province, China
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辽宁省宫颈病变女性人乳头瘤病毒58型E6、E7、L1基因及长控制区变异
DOI：
10.1016/j.meegid.2012.05.004
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发表时间：
2012-10-01
影响因子：
3.2
通讯作者：
Ruan, Qiang
中科院分区：
文献类型：
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作者：
Liu, Jian-hua;Lu, Zhi-tao;Ruan, Qiang
感染某些类型的人乳头瘤病毒 (HPV) 是患宫颈癌的危险因素。 HPV 58 型（HPV 58）在中国女性中流行。类型内序列变异的致癌潜力不同，其患病率在不同地理区域也不同。本研究的目的是分析中国东北宫颈病变女性的大样本中HPV 58 E6、E7、L1基因和长控制区（LCR）的变异。总共收集了 2938 个宫颈样本，并使用芯片杂交检测检测 HPV 类型。扩增HPV 58毒株的E6、E7、L1基因和LCR，并对扩增子进行直接核苷酸测序以进行变异鉴定。共有 235 份标本呈 HPV 58 阳性。在中国女性的毒株中发现了高比例的 HPV 58 E6 (83.8%)、E7 (76.7%)、L1 (90.8%) 基因和 LCR (91.4%) 变异。最常见的变异是 E6 中的 C307T (52.4%)、E7 中的 T744G (74.9%)、L1 基因中的 A6014C (56.9%) 以及 LCR 中的 C7266T、A7714G (55.2%)。对于 E6 基因，鉴定出九个核苷酸变异。其中，A140G（T11A）、A184C（E25D）、G266C（V53L）和A313G是新颖的变体。 E7基因的测序揭示了四种典型的核苷酸变化：G761A (G63D)、G694A (G41R)、T803C (V77A)和T744G。在 L1 基因中，鉴定出 39 个核苷酸变异和 13 个氨基酸取代。在这些突变中，有 21 个变异是首次报道。在 174 株毒株中的 142 株中发现了 HPV 58 谱系 A (81.6%)。中国东北地区女性中最流行的HPV 58变异属于A谱系。E6和L1基因也有新的变异报道。这些发现提供了有关中国东北地区女性 HPV 58 E6 和 L1 基因变异的新数据。 (c) 2012 Elsevier B.V. 保留所有权利。
Infection with certain types of human papillomaviruses (HPVs) is a risk factor for the development of cervical cancer. HPV type 58 (HPV 58) is prevalent among Chinese women. The intratype sequence variants differ in oncogenic potential and their prevalences vary across geographic regions. The objective of this study was to analyze the variations of HPV 58 E6, E7, L1 genes and long control region (LCR) in a large samples collected from northeastern Chinese women with cervical lesions. A total of 2938 cervical samples were collected and tested for HPV type using a chip hybridization assay. The E6, E7, L1 genes and LCR of HPV 58 strains were amplified and the amplicons were subjected to direct nucleotide sequencing for variation identification. A total of 235 specimens were HPV 58 positive. High proportions of HPV 58 E6 (83.8%), E7 (76.7%), L1 (90.8%) genes and LCR (91.4%) variants were identified in strains from Chinese women. The most frequently observed variations were C307T (52.4%) in E6, T744G (74.9%) in E7, A6014C (56.9%) in L1 genes and C7266T, A7714G (55.2%) in LCR. For the E6 gene, nine nucleotide variations were identified. Among them, the A140G (T11A), A184C (E25D), G266C (V53L) and A313G were novel variations. Sequencing of the E7 gene revealed four typical nucleotide changes: G761A (G63D), G694A (G41R), T803C (V77A) and T744G. In the L1 gene, 39 nucleotide variations and 13 amino acid substitutions were identified. Among these mutations, 21 variations are reported here for the first time. Lineage A of HPV 58 was found in 142 of 174 strains (81.6%). The most prevalent HPV 58 variants in Chinese northeastern women belongs to lineage A. Novel variations in E6 and L1 genes were also reported. These findings provide new data regarding E6 and L1 gene variations of HPV 58 from women in northeast China. (c) 2012 Elsevier B.V. All rights reserved.