Electrical stimulation enhances neuronal cell activity mediated by Schwann cell derived exosomes
Electrical stimulation enhances neuronal cell activity mediated by Schwann cell derived exosomes
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电刺激增强雪旺细胞衍生的外泌体介导的神经细胞活性
DOI：
10.1038/s41598-019-41007-5
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发表时间：
2019-03-12
影响因子：
4.6
通讯作者：
Chen, Qian
中科院分区：
文献类型：
--
作者：
Hu, Ming;Hong, Li;Chen, Qian
电刺激治疗对神经系统损伤相关疾病有较好的疗效。ES促进神经细胞再生并刺激雪旺细胞表达神经营养因子。压力性尿失禁（SUI）在老年人中的发病率正在增加。有研究认为阴部神经的损伤与SUI的发病密切相关。还发现盆腔ES可以减轻由阴部神经损伤引起的SUI大鼠模型中的SUI症状。临床上，盆底电刺激对轻度至中度SUI患者有效。这些研究表明，ES可以改善阴部神经的损伤，从而达到SUI治疗的目的，尽管这种治疗的作用机制尚不清楚。因此，本研究的目的是从细胞水平上阐明胚胎干细胞、神经细胞和雪旺细胞之间的关系。我们将ES以100 mV/mm或200 mV/mm施加于神经细胞0、0.5、1或2 h，以研究神经细胞活性的变化。然后将神经细胞与雪旺细胞共培养，探讨单一培养和共培养条件对神经细胞的影响。与非ES相比，ES的神经细胞的活性增加。与单一培养相比，共培养的神经细胞表现出额外的活性增加。我们还发现雪旺细胞来源的外泌体可以促进神经细胞的活性，谷氨酸和钙离子在这个过程中发挥了潜在的作用。这些结果提示神经细胞和雪旺细胞的相互调节在ES改善神经功能的过程中起着重要作用，为后续的研究提供了依据。
Electrical stimulation (ES) therapy has good effects in patients with nervous system injury-related diseases. ES promotes nerve cell regeneration and stimulates Schwann cells to express neurotrophic factors. The incidence of stress urinary incontinence (SUI) among elderly people is increasing. Some studies suggest that damage to the pudendal nerve is closely related to the pathogenesis of SUI. It has also been found that pelvic ES can reduce SUI symptoms in a rat model of SUI caused by pudendal nerve injury. Clinically, pelvic floor electrical stimulation is effective in patients with mild to moderate SUI. These studies indicate that ES may ameliorate damage to the pudendal nerve and thus achieve the goal of SUI treatment, although the mechanism of action of this treatment remains unclear. Therefore, the purpose of the present study was to clarify the relationships among ES, neural cells and Schwann cells at the cellular level. We applied ES to nerve cells at 100 mV/mm or 200 mV/mm for 0, 0.5, 1, or 2 h to investigate changes in nerve cell activity. We then co-cultured the nerve cells with Schwann cells to explore the influence of single-culture and co-culture conditions on the nerve cells. Compared to non-ES, ES of the nerve cells increased their activity. Compared to those in single culture, co-cultured nerve cells exhibited an additional increase in activity. We also found that Schwann cell derived exosomes could promote the activity of nerve cells, with glutamate and calcium ions playing a potential role in this process. These results suggest that the mutual regulation of neural cells and Schwann cells plays an important role in the process by which ES ameliorates neurological function, which may provide a basis for subsequent studies.