Deaggregation of Human Platelets Aggregated by Thrombin
Deaggregation of Human Platelets Aggregated by Thrombin
复制标题
凝血酶聚集的人血小板的解聚
DOI：
10.1055/s-0038-1661233
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发表时间：
1985
影响因子：
6.7
通讯作者：
J. Mustard
中科院分区：
文献类型：
--
作者：
R. Kinlough;D. Perry;M. Packham;J. Mustard
经过释放反应的人血小板不容易解聚。我们研究了在凝血酶(1或5U/ml)诱导的广泛释放反应后，洗涤的人血小板可以解聚的条件。为了使纤维蛋白原受体不可用，要么使用CP/CPK(或apyrase)来清除释放的ADP，要么使用PGEi来增加cAMP。胰凝乳素被用来消化可能连接血小板的蛋白质，肝素被用来与释放的蛋白质相互作用，并干扰它们与血小板和相互之间的结合。单独来看，这些都没有引起解聚；肝素不能抑制凝血酶的作用，因为不存在抗凝血酶III。在凝血酶(1U/ml)作用下，经水飞蓟素中和90s后，CP/CPK(或apyrase)和胰凝乳酶或前列腺素E_1(PGE_1)和胰凝乳酶的联合作用可使血小板解聚。当使用较高浓度的凝血酶(5U/ml)时，只有在凝血酶诱导释放反应之前加入肝素时，这些组合才会导致血小板解聚。因此，当发生广泛的释放时，三种机制可能发挥作用来连接人类血小板：一种需要纤维蛋白原受体；一种可能涉及释放的蛋白质结合的肝素敏感反应；以及一种只有蛋白分解才能破坏的连接，前提是另外两种机制也被抑制。
Summary Human platelets that have undergone the release reaction do not deaggregate readily. We examined conditions under which washed human platelets can be deaggregated after they have undergone an extensive release reaction induced by thrombin (1 or 5 U/ml). To make fibrinogen receptors unavailable, either CP/ CPK (or apyrase) was used to remove released ADP, or PGEi was used to increase cAMP. Chymotrypsin was used to digest proteins that might link platelets, and heparin to interact with released proteins and interfere with their binding to platelets and to each other. Individually, none of these caused deaggregation; heparin did not inhibit the effect of thrombin because no antithrombin III was present. Platelets exposed to thrombin (1 U/ ml) which was neutralized at 90 sec by hirudin, could be deaggregated by combinations of CP/CPK (or apyrase) and chymotrypsin, or PGE1 and chymotrypsin. When a higher concentration of thrombin was used (5 U/ml) these combinations caused platelets to deaggregate only when heparin was added before thrombin induced the release reaction. Thus, when extensive release occurs three mechanisms may come into play to link human platelets: one that requires the fibrinogen receptor; a heparin-sensitive reaction that may involve the binding of released proteins; and a linkage that can be disrupted only by proteolysis, providing the other two mechanisms are also inhibited.
富含组氨酸的糖蛋白存在于人血小板中，并在凝血酶刺激后释放。
影响因子：
20.3
作者：
Leung,LL;Harpel,PC;Nachman,RL;Rabellino,EM
通讯作者：
Rabellino,EM