The relationship of quantitative nuclear morphology to molecular genetic alterations in the adenoma-carcinoma sequence of the large bowel.
The relationship of quantitative nuclear morphology to molecular genetic alterations in the adenoma-carcinoma sequence of the large bowel.
复制标题
DOI：
--
复制
发表时间：
1992-10
期刊：
影响因子：
--
通讯作者：
J. Mulder;G. Offerhaus;E. D. de Feyter;J. Floyd;S. Kern;B. Vogelstein;S. Hamilton
中科院分区：
文献类型：
--
作者：
J. Mulder;G. Offerhaus;E. D. de Feyter;J. Floyd;S. Kern;B. Vogelstein;S. Hamilton
异常核形态与结直肠肿瘤发生中重要的分子遗传学改变的关系尚不清楚。因此，Feulgen染色的22个腺瘤和42个癌细胞核的ras基因突变和染色体5 q，18 q和17 p上的等位基因缺失进行了分析，其特征在于计算机图像分析。核面积和核形状因子均与腺瘤-癌的进展相关（r = 0.57和r = 0.52，P < 0.0001），而标准核质地（染色质同质性参数）与进展呈负相关（r =-0.80，P < 0.0001）。各核参数之间存在高度相关性（P < 0.0005）。在多变量分析中，核参数主要与腺瘤-癌进展相关（P <或= 0.0001），而不受个体分子遗传学改变的显著影响。然而，在癌中，核纹理与等位基因丢失分数（一种遗传变化的总体测量）呈负相关（r =-0.39，P = 0.011）。研究结果表明，结直肠肿瘤的核形态与肿瘤进展密切相关。核形态和生物学行为似乎受到癌症相关基因累积改变的影响。
The relationship of abnormal nuclear morphology to molecular genetic alterations that are important in colorectal tumorigenesis is unknown. Therefore, Feulgen-stained isolated nuclei from 22 adenomas and 42 carcinomas that had been analyzed for ras gene mutations and allelic deletions on chromosomes 5q, 18q, and 17p were characterized by computerized image analysis. Both nuclear area and the nuclear shape factor representing irregularity correlated with adenoma-carcinoma progression (r = 0.57 and r = 0.52, P < 0.0001), whereas standard nuclear texture, a parameter of chromatin homogeneity, was inversely correlated with progression (r = -0.80, P < 0.0001). The nuclear parameters were strongly interrelated (P < 0.0005). In multivariate analysis, the nuclear parameters were predominantly associated with adenoma-carcinoma progression (P < or = 0.0001) and were not influenced significantly by the individual molecular genetic alterations. Nuclear texture, however, was inversely correlated with fractional allelic loss, a global measure of genetic changes, in carcinomas (r = -0.39, P = 0.011). The findings indicate that nuclear morphology in colorectal neoplasms is strongly related to tumor progression. Nuclear morphology and biologic behavior appear to be influenced by accumulated alterations in cancer-associated genes.