Bioactivity of periodontal ligament stem cells on sodium titanate coated with graphene oxide.
Bioactivity of periodontal ligament stem cells on sodium titanate coated with graphene oxide.
复制标题
氧化石墨烯包覆钛酸钠对牙周膜干细胞的生物活性
DOI：
10.1038/srep19343
复制
发表时间：
2016-01-14
影响因子：
4.6
通讯作者：
Ge S
中科院分区：
文献类型：
--
作者：
Zhou Q;Yang P;Li X;Liu H;Ge S
氧化石墨烯（graphene oxide，GO）作为一种生物相容性好、细胞毒性低的纳米材料，在组织工程领域引起了人们极大的兴趣。然而，关于GO上的牙齿干细胞的行为知之甚少。本研究旨在比较人牙周膜干细胞（PDLSCs）在GO涂层钛（GO-Ti）和钛酸钠（Na-Ti）基质上的生物活性。通过扫描电镜（SEM）、激光共聚焦扫描显微镜（CLSM）、四甲基偶氮唑盐（MTT）比色法、碱性磷酸酶（ALP）活性测定、实时定量聚合酶链反应（qRT-PCR）和western blot分析，观察PDLSCs在两种基质上的附着、形态、增殖和成骨分化情况。与Na-Ti基质相比，GO-Ti基质上PDLSCs的增殖率、ALP活性及成骨相关标志物Ⅰ型胶原（COL-I）、ALP、骨涎蛋白（BSP）、Runx 2和骨钙素（OCN）的基因表达水平均显著升高。GO还促进BSP、Runx 2和OCN蛋白的表达。这些研究结果表明，GO和PDLSCs的组合为再生牙科提供了一个有前途的构建体。
As a biocompatible and low cytotoxic nanomaterial, graphene oxide (GO) has captured tremendous interests in tissue engineering. However, little is known about the behavior of dental stem cells on GO. This study was to evaluate the bioactivity of human periodontal ligament stem cells (PDLSCs) on GO coated titanium (GO-Ti) substratein vitroas compared to sodium titanate (Na-Ti) substrate. By scanning electron microscope (SEM), confocal laser scanning microscope (CLSM), methylthiazol tetrazolium (MTT) assay, alkaline phosphatase (ALP) activity, quantitative real-time polymerase chain reaction (qRT-PCR) and western blot analysis, we investigated the attachment, morphology, proliferation and osteogenic differentiation of PDLSCs on these two substrates. When seeded on GO-Ti substrate, PDLSCs exhibited significantly higher proliferation rate, ALP activity and up-regulated gene expression level of osteogenesis-related markers of collagen type I (COL-I), ALP, bone sialoprotein (BSP), runt related transcription factor 2 (Runx2) and osteocalcin (OCN) compared with those on Na-Ti substrate. Moreover, GO promoted the protein expression of BSP, Runx2 and OCN. These findings suggest that the combination of GO and PDLSCs provides a promising construct for regenerative dentistry.