Tissue refractometry using Hilbert phase microscopy
Tissue refractometry using Hilbert phase microscopy
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DOI：
10.1364/ol.32.003522
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发表时间：
2007-12-15
期刊：
影响因子：
3.6
通讯作者：
Popescu, Gabriel
中科院分区：
文献类型：
--
作者：
Lue, Niyom;Bewersdorf, Joerg;Popescu, Gabriel
我们提出，第一次我们的知识，定量相位图像与未染色的5 μ m厚的组织切片的小鼠脑，脾，肝。根据平均折射率及其空间变化来检索组织的折射特性。我们发现，平均折射率随组织类型而显著变化，使得大脑的特征在于最低值，而肝脏的特征在于最高值。相位图像的空间功率谱揭示了每种组织类型具有不同指数的幂律行为。这种方法为组织的无染色表征开辟了新的可能性，其中诊断能力由生物结构的固有折射特性提供。我们目前获得的结果为肝组织受溶酶体贮积病，并表明，我们的技术可以量化这种疾病的发展过程中的结构变化。(c)2007年，美国光学学会。
We present, for the first time to our knowledge, quantitative phase images associated with unstained 5 mu m thick tissue slices of mouse brain, spleen, and liver. The refractive properties of the tissue are retrieved in terms of the average refractive index and its spatial variation. We find that the average refractive index varies significantly with tissue type, such that the brain is characterized by the lowest value and the liver by the highest. The spatial power spectra of the phase images reveal power law behavior with different exponents for each tissue type. This approach opens a new possibility for stain-free characterization of tissues, where the diagnostic power is provided by the intrinsic refractive properties of the biological structure. We present results obtained for liver tissue affected by a lysosomal storage disease and show that our technique can quantify structural changes during this disease development. (c) 2007 Optical Society of America.