CD4+ T-cell immunity to the Burkholderia pseudomallei ABC transporter LolC in melioidosis.
CD4+ T-cell immunity to the Burkholderia pseudomallei ABC transporter LolC in melioidosis.
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DOI：
10.1002/eji.201040881
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发表时间：
2011-01
影响因子：
5.4
通讯作者：
Altmann, Daniel M.
中科院分区：
文献类型：
--
作者：
Chu, Karen K.;Tippayawat, Patcharaporn;Walker, Nicola J.;Harding, Sarah V.;Atkins, Helen S.;Maillere, Bernard;Bancroft, Gregory J.;Lertmemongkolchai, Ganjana;Altmann, Daniel M.
Burkholderia pseudomallei (Bp) causes melioidosis, a disease with a wide range of possible outcomes, from seroconversion and dormancy to sepsis and death. This spectrum of host-pathogen interactions poses challenging questions about heterogeneity in immunity to Bp. Models show protection to be dependent on CD4+ cells and IFNγ, but little is known about specific target antigens. Having previously implicated the ABC transporter, LolC, in protective immunity, we here use epitope prediction, HLA binding studies, HLA-transgenic models and studies of T cells from seropositive individuals to characterize HLA-restricted LolC responses. Immunized mice showed long-lasting memory to the protein, while predictive algorithms identified epitopes within LolC that subsequently demonstrated strong HLA class II binding. Immunization of HLA-DR transgenics with LolC stimulated T cell responses to four of these epitopes. Furthermore, responsiveness of HLA-transgenics to LolC revealed a hierarchy supportive of HLA polymorphism-determined differential susceptibility. Seropositive human donors of diverse HLA class II types showed T cell responses to LolC epitopes which are conserved among Burkholderia species including B. cenocepacia, associated with life-threatening cepacia complex in cystic fibrosis patients and B. mallei, which causes glanders. These findings suggest a role for LolC epitopes in multiepitope vaccine design for melioidosis and related diseases.
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影响因子：
2.7
作者：
Dharakul, T;Vejbaesya, S;Songsivilai, S
通讯作者：
Songsivilai, S
DOI：
10.4269/ajtmh.2005.73.1165
发表时间：
2005-12-01
影响因子：
3.3
作者：
Chierakul, W;Wuthiekanun, V;Peacock, SJ
通讯作者：
Peacock, SJ
DOI：
10.1084/jem.183.6.2635
发表时间：
1996-06-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Ito K;Bian HJ;Molina M;Han J;Magram J;Saar E;Belunis C;Bolin DR;Arceo R;Campbell R;Falcioni F;Vidović D;Hammer J;Nagy ZA
通讯作者：
Nagy ZA
DOI：
10.1084/jem.181.3.867
发表时间：
1995-03-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Altmann DM;Douek DC;Frater AJ;Hetherington CM;Inoko H;Elliott JI
通讯作者：
Elliott JI
影响因子：
4.4
作者：
Ingram, Rebecca J.;Metan, Goekhan;Altmann, Daniel M.
通讯作者：
Altmann, Daniel M.