Distribution and expression of protein kinase c interactive protein (PKCI/HINT1) in mouse central nervous system (CNS)
Distribution and expression of protein kinase c interactive protein (PKCI/HINT1) in mouse central nervous system (CNS)
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DOI：
10.1007/s11064-007-9578-4
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发表时间：
2008-07-01
影响因子：
4.4
通讯作者：
Wang, Jia Bei
中科院分区：
文献类型：
--
作者：
Liu, Qing;Puche, Adam C.;Wang, Jia Bei
蛋白激酶C相互作用蛋白（PKCI;也称为组氨酸三联体蛋白，HINT 1）是一种在外周和CNS组织中广泛表达的小细胞内蛋白。虽然该蛋白的结构已被很好地表征，但该蛋白的功能方面和细胞分布仍然未知，特别是在CNS中。为了分析PKCI/HINT 1的表达模式，我们使用了针对完整重组蛋白或PKCI/HINT 1的肽表位的抗体。我们通过Western blot和免疫组化发现PKCI/HINT 1在小鼠中枢神经系统中广泛表达。我们的数据表明PKCI/HINT 1广泛存在于中枢神经系统的各个区域，在嗅觉系统、大脑皮层、海马和部分丘脑、下丘脑、中脑、脑桥和延髓中相对较高的丰度。在细胞水平上，PKCI/HINT 1免疫反应性主要位于神经元和神经元过程中。本研究为PKCI/HINT 1在神经元功能中的潜在作用提供了解剖学证据。
Protein kinase C interactive protein (PKCI; also known as histidine triad protein, HINT1) is a small intracellular protein widely expressed in tissues from both the peripheral and CNS. Although the structure of this protein is well characterized, the functional aspect and cellular distribution of the protein remain unknown, especially in CNS. To analyze the expression pattern of PKCI/HINT1 we used antibodies against either the whole recombinant protein or a peptide epitope of PKCI/HINT1. We find widespread of PKCI/HINT1 expression in the mouse CNS by Western blot and immunostaining. Our data indicates that PKCI/HINT1 is present broadly throughout the regions of CNS with relatively high abundance in olfactory system, cerebral cortex, hippocampus and part of thalamus, hypothalamus, midbrain, pons and medulla. On the cellular level, PKCI/HINT1 immunoreactivity is primarily located in neurons and neuronal processes. This study provides the anatomical evidence for the potential roles of PKCI/HINT1 in neuronal function.