Production of bioemulsifiers by Amycolatopsis tucumanensis DSM 45259 and their potential application in remediation technologies for soils contaminated with hexavalent chromium
Production of bioemulsifiers by Amycolatopsis tucumanensis DSM 45259 and their potential application in remediation technologies for soils contaminated with hexavalent chromium
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DOI：
10.1016/j.jhazmat.2013.08.005
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发表时间：
2013-10-15
影响因子：
13.6
通讯作者：
Beatriz Villegas, Liliana
中科院分区：
文献类型：
--
作者：
Leticia Colin, Veronica;Fernanda Castro, Maria;Beatriz Villegas, Liliana
近年来，人们对使用生物乳化剂作为清洗剂越来越感兴趣，该清洗剂可以增强土壤结合金属的解吸。然而，由于使用昂贵的底物来配制发酵培养基而产生的高生产成本是全面、大规模实施生物乳化剂的主要挑战。这项工作报告了放线杆菌土库曼无枝酸菌 DSM 45259 使用不同碳源和氮源生产生物乳化剂的首次研究。还介绍了这些化合物作为被 Cu(II) 和 Cr(VI) 污染的土壤的洗涤剂的潜在用途的初步结果。分别使用甘油和尿素作为碳和氮底物检测到最佳比产量。然而，对于批量测定过程中使用的所有底物，生物乳化剂在 pH、温度和盐浓度的极端条件下表现出高水平的稳定性。在目前的测定条件下，生物乳化剂不能有效去除土壤中的 Cu(II)。然而，他们能够介导 Cr(VI) 回收，去除百分比比使用去离子水时增加了一倍。这些发现对于开发基于直接使用这些微生物乳化剂的六价铬化合物修复技术来说似乎很有希望。 (C) 2013 Elsevier B.V. 保留所有权利。
In recent years, increasing interest has been shown in the use of bioemulsifiers as washing agents that can enhance desorption of soil-bound metals. However, high production costs derived from the use of expensive substrates for formulation of the fermentation media represent the main challenge for full, large-scale implementation of bioemulsifiers. This work reports on a first study of bioemulsifier production by the actinobacterium Amycolatopsis tucumanensis DSM 45259 using different carbon and nitrogen sources. Preliminary results on the potential use of these compounds as washing agents for soils contaminated with Cu(II) and Cr(VI) are also presented. The best specific production was detected using glycerol and urea as carbon and nitrogen substrates, respectively. However, with all of the substrates used during the batch assay, the bioemulsifiers showed high levels of stability at extreme conditions of pH, temperature, and salt concentration. Under the current assay conditions, the bioemulsifiers were not effective in removing Cu(II) from soil. However, they were able to mediate Cr(VI) recovery, with the removal percentage doubled compared to that seen when using deionized water. These findings appear promising for the development of remediation technologies for hexavalent chromium compounds based upon direct use of these microbial emulsifiers. (C) 2013 Elsevier B.V. All rights reserved.