Lung cancer neovascularisation: Cellular and molecular interaction between endothelial and lung cancer cells
Lung cancer neovascularisation: Cellular and molecular interaction between endothelial and lung cancer cells
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DOI：
10.1016/j.imbio.2013.11.004
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发表时间：
2014-04-01
期刊：
影响因子：
2.8
通讯作者：
Plendl, Johanna
中科院分区：
文献类型：
--
作者：
Kaessmeyer, Sabine;Bhoola, Kanti;Plendl, Johanna
背景：在一些癌症中已经观察到了新的不依赖于血管的管道。这些已经被不同地描述为血管生成拟态，马赛克血管形成，血管共选择和肿瘤内胚胎样血管发生。尽管肺癌是世界上最常见的癌症，有很少的信息，其新生血管形成或pathways involved.Methods：一个体外模型，涉及微血管肺内皮细胞和肺鳞癌或腺癌细胞的共培养，以评估其血管生成的相互作用。在单培养物和共培养物中孵育细胞，并通过相差显微镜和免疫细胞化学进行检查。培养的细胞和肺癌组织切片进行了评估新的肿瘤血管形成，表达的内皮标志物CD31和morphology.Results：肺肿瘤细胞和内皮细胞相互作用的形态通过伪足和使用替代途径产生新的血管。共培养微血管内皮细胞和鳞状细胞癌细胞导致内皮细胞周围的肿瘤细胞和肿瘤细胞被纳入血管壁。共培养内皮细胞和腺癌细胞导致细胞接触，并在内皮细胞簇周围形成肿瘤细胞桥。这些腺癌细胞成为CD31强阳性。肿瘤组织切片研究支持在体外findings.Conclusion：肺癌细胞与肺内皮细胞共培养时，修改其细胞和分子特征，鼓励替代手段提供血液供应。这些非血管生成过程的机制需要进一步研究，并应考虑在考虑抗肿瘤治疗干预措施。(C)2013 Elsevier GmbH. All rights reserved.
Background: Novel vascular-independent conduits have been observed in some cancers. These have been variously described as vasculogenic mimicry, mosaic vessel formation, vascular co-option and intra-tumour embryonic-like vasculogenesis. Despite lung cancer being the most common cancer worldwide, there is little information on its neovascularisation or the pathways involved.Methods: An in vitro model involving co-cultures of microvascular lung endothelial cells and squamous or adenocarcinoma lung cancer cells was developed to assess their angiogenic interaction. Cells were incubated and examined by phase contrast microscopy and by immunocytochemistry in both mono- and co-cultures. Cultured cells and lung cancer tissue sections were assessed for new tumour vessel formation, expression of the endothelial marker CD31 and morphology.Results: Lung tumour cells and endothelial cells interacted morphologically via pseudopodia and used alternative pathways to generate new vessels. Co-culturing microvascular endothelial and squamous carcinoma cells led to endothelial cells surrounding tumour cells and the tumour cells being incorporated into vessel walls. Co-culturing endothelial and adenocarcinoma cells resulted in cellular contact and the formation of tumour cell bridges around clusters of endothelial cells. These adencocarcinoma cells became strongly positive for CD31. Tumour tissue section studies supported the in vitro findings.Conclusion: Lung carcinoma cells when co-cultured with lung endothelial cells modify their cellular and molecular features that encourage alternative means of providing blood supply. The mechanisms under-pinning these non-angiogenic processes need to be further investigated and should be considered when anti-tumour therapeutic interventions are being considered. (C) 2013 Elsevier GmbH. All rights reserved.