Type 1 diabetes cadaveric human pancreata exhibit a unique exocrine tissue proteomic profile.
Type 1 diabetes cadaveric human pancreata exhibit a unique exocrine tissue proteomic profile.
复制标题
DOI：
10.1002/pmic.201500333
复制
发表时间：
2016-05
期刊：
影响因子：
3.4
通讯作者：
Zhang Q
中科院分区：
文献类型：
--
作者：
Liu CW;Atkinson MA;Zhang Q
1型糖尿病（T1D）是一种由胰岛细胞自我破坏引起的自身免疫性疾病。人类胰腺的完整蛋白质组，其中功能失调的β细胞和其近端环境共存，仍然是未知的。在这里，我们使用基于tmt10的等压标记和多维LC-MS/MS定量分析了T1D （N = 5）和健康受试者（N = 5）胰腺头部区域组织之间的差异。在5357个（1%的错误发现率）确定的蛋白中，145个在T1D和健康受试者之间显示有统计学意义的失调。差异表达的胰腺蛋白质组支持了外分泌胰腺参与T1D的潜在作用这一日益增长的概念。这项研究也证明了这种方法的实用性，分析失调蛋白作为一种手段来研究胰岛生物学，胰腺病理和T1D发病机制。
Type 1 diabetes (T1D) is an autoimmune disorder resulting from a self-destruction of pancreatic islet beta cells. The complete proteome of the human pancreas, where both the dysfunctional beta cells and their proximal environment co-exist, remains unknown. Here, we used TMT10-based isobaric labeling and multidimensional LC-MS/MS to quantitatively profile the differences between pancreatic head region tissues from T1D (N = 5) and healthy subjects (N = 5). Among the 5357 (1% false discovery rate) confidently identified proteins, 145 showed statistically significant dysregulation between T1D and healthy subjects. The differentially expressed pancreatic proteome supports the growing notion of a potential role for exocrine pancreas involvement in T1D. This study also demonstrates the utility for this approach to analyze dysregulated proteins as a means to investigate islet biology, pancreatic pathology and T1D pathogenesis.