Anxiolytic-like effects of restraint during the dark cycle in adolescent mice
Anxiolytic-like effects of restraint during the dark cycle in adolescent mice
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DOI：
10.1016/j.bbr.2015.02.010
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发表时间：
2015-05-01
影响因子：
2.7
通讯作者：
Matsuda, Toshio
中科院分区：
文献类型：
--
作者：
Ota, Yuki;Ago, Yukio;Matsuda, Toshio
发育阶段的应激可能导致心理疾病，因此对青春期啮齿动物应激的研究具有重要意义。约束被用作啮齿动物的常见应激源，并且已经研究了在光周期期间约束的影响，但是在暗周期期间约束的影响还没有。本研究探讨了在光照和黑暗周期中的约束对青春期小鼠焦虑行为的影响。在光照或黑暗周期中束缚3小时会损害恐惧条件反射测试中的记忆功能，但不影响运动活动。在高架十字迷宫测试中，在黑暗周期中的束缚减少了小鼠的焦虑样行为。重复暴露于3小时的黑暗周期约束2周有类似的抗焦虑样作用。相比之下，约束3小时，在光周期产生焦虑行为的青少年，但不是成年小鼠。光周期应激增加血浆皮质酮水平，增加前额叶皮质、下丘脑室旁核、杏仁基底外侧核和齿状回c-Fos表达，增加海马和纹状体5-羟色胺周转，而暗周期应激则无此作用。在黑暗和光照周期限制之间，戊巴比妥诱导的睡眠时间的应激介导的减少没有差异。这些发现表明，暗循环束缚的抗焦虑作用是由皮质酮、5-羟色胺或γ-氨基丁酸非依赖性机制介导的，尽管光循环束缚的致焦虑作用与血浆皮质酮水平和特定脑区5-羟色胺周转的变化有关。(C)2015 Elsevier B. V.版权所有。
Stress during developmental stage may cause psychological morbidities, and then the studies on stress are important in adolescent rodents. Restraint is used as a common stressor in rodents and the effects of restraint during the light cycle have been studied, but those of restraint during the dark cycle have not. The present study examined the effects of restraint during the light and dark cycles on anxiety behaviors in adolescent mice. Restraint for 3 h during either the light or dark cycle impaired memory function in the fear conditioning test, but did not affect locomotor activity. In the elevated plus-maze test, restraint during the dark cycle reduced anxiety-like behaviors in mice. Repeated exposure to a 3-h period dark cycle restraint for 2 weeks had a similar anxiolytic-like effect. In contrast, restraint for 3 h during the light cycle produced anxiety behavior in adolescent, but not adult, mice. The light cycle stress increased plasma corticosterone levels, and elevated c-Fos expression in the prefrontal cortex, paraventricular hypothalamic nucleus, basolateral amygdala and dentate gyrus, and enhanced serotonin turnover in the hippocampus and striatum, while the dark cycle stress did not. There was no difference in the stress-mediated reduction in pentobarbital-induced sleeping time between dark and light cycle restraint. These findings suggest that the anxiolytic effect of dark cycle restraint is mediated by corticosterone, serotonin or "y-aminobutyric acid-independent mechanisms, although the anxiogenic effect of light cycle restraint is associated with changes in plasma corticosterone levels and serotonin turnover in specific brain regions. (C) 2015 Elsevier B.V. All rights reserved.