Sequence analysis of myostatin promoter in cattle
Sequence analysis of myostatin promoter in cattle
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DOI：
10.1159/000075724
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发表时间：
2003-01-01
影响因子：
1.7
通讯作者：
Valentini, A
中科院分区：
文献类型：
--
作者：
Crisà, A;Marchitelli, C;Valentini, A
肌肉生长抑制素（GDF8）是肌肉生长的负调节因子。该基因的突变导致了几个欧洲牛品种的双重肌肉表型。这里我们描述了肌生长抑制素基因上游5'区域的序列。对欧洲9个牛品种的3只动物进行了序列分析，目的是寻找多态性。T/A多态性在-371，G/C多态性在-805（相对于ATG）。利用PCR-RFLP进一步筛选9个品种的353只动物，并评估snp的频率。该基因的启动子区域包含几个转录因子的结合位点，这些转录因子也在其他肌源性基因中发现。这可能在蛋白质的调节中发挥重要作用，从而影响肌肉的发育。版权所有(C) 2003 S. Karger AG，巴塞尔。
Myostatin (GDF8) acts as a negative regulator of muscle growth. Mutations in the gene are responsible for the double muscling phenotype in several European cattle breeds. Here we describe the sequence of the upstream 5' region of the myostatin gene. The sequence analysis was carried out on three animals of nine European cattle breeds, with the aim to search for polymorphisms. A T/A polymorphism at -371 and a G/C polymorphism at -805 (relative to ATG) were found. PCR-RFLP was used to further screen 353 animals of the nine breeds studied and to assess the frequencies of the SNPs. The promoter region of the gene contains several binding sites for transcription factors found also in other myogenic genes. This may play an important role in the regulation of the protein and consequently on muscular development. Copyright (C) 2003 S. Karger AG, Basel.