Viral infections in common carp lead to a disturbance of mucin expression in mucosal tissues
Viral infections in common carp lead to a disturbance of mucin expression in mucosal tissues
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DOI：
10.1016/j.fsi.2017.10.029
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发表时间：
2017-12-01
影响因子：
4.7
通讯作者：
Steinhagen, Dieter
中科院分区：
文献类型：
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作者：
Adamek, Mikolaj;Hazerli, Dennis;Steinhagen, Dieter
为了应对潜在病原体的持续攻击，鱼的外表面，即它们的皮肤、鳃和肠道，都覆盖着粘液，这是一种凝胶状物质，在很大程度上阻止了病原体的进入。这种粘液凝胶主要由水和粘蛋白组成，这是一种大的O-糖基化蛋白质，它们负责形成凝胶状混合物。在哺乳动物的病毒性疾病中，粘蛋白的mRNA表达被描述为一种调节，但在鱼类病毒感染过程中，对粘蛋白的调节还存在一个知识空白。因此，鲤鱼粘蛋白的新序列位于鲤鱼基因组的早期版本，并在鲤鱼体内检测到它们在三种不同病毒病原体感染下的mRNA表达：(I)异基因疱疹病毒鲤鱼疱疹病毒3型，(Ii)鲤鱼病毒的弹状病毒春季病毒血症和(Iii)痘病毒鲤鱼水肿症病毒。结果表明，在这些病原病毒感染下，鲤鱼的鳃和肠道中粘蛋白mRNA的表达下调。这可能是鲤鱼在病毒感染下粘膜组织严重受损的迹象。粘蛋白表达的减少可能有助于解释感染病毒的鲤鱼对继发性细菌感染的易感性增加。
In response to the constant challenge by potential pathogens, external surfaces of fish, their skin, gills and intestinal tract, are coated with mucus, a gel like substance which largely prevents the entry of pathogens. This mucus gel consists mainly of water and mucins, large O-glycosylated proteins, which are responsible for forming a gel like mixture. A modulation of the mRNA expression of mucins, was described in viral diseases in mammals however there is a knowledge gap about the regulation of mucins during viral infection in fish. Therefore, novel sequences for common carp mucins were located in an early version of the common carp genome and their mRNA expression measured in carp under infection with three different viral pathogens: (i) the alloherpesvirus cyprinid herpesvirus 3, (ii) the rhabdovirus spring viremia of carp virus and (iii) the poxvirus carp edema virus. The results showed a downregulation of mucin mRNA expression in gills and gut of common carp under infection with these pathogenic viruses. This could be a sign of a severe distress to the mucosal tissues in carp which occurs under viral infection. The reduced expression of mucins could help explaining the increased susceptibility of virus-infected carp to secondary bacterial infection.