Melanocytic differentiation of human neuroblastoma: expression of a human melanosome-associated antigen.
Melanocytic differentiation of human neuroblastoma: expression of a human melanosome-associated antigen.
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人神经母细胞瘤的黑素细胞分化：人黑素体相关抗原的表达。
DOI：
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发表时间：
1990
影响因子：
6.5
通讯作者：
L. Jerry
中科院分区：
文献类型：
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作者：
K. Maeda;V. Feyles;R. Mcgarry;L. Jerry
我们检测了5种人神经母细胞瘤细胞系中人黑色素体相关抗原（HMSA）的表达。只有细胞系SK-N-SH与单克隆抗体HMSA-2反应，显示识别黑素体糖蛋白。为了进一步表征SK-N-SH的黑素细胞谱系，通过集落形成克隆建立了三个形态不同的克隆，分别是SK-N-SH- n（神经母细胞型）、SK-N-SH- f（成纤维细胞型）和SK-N-SH- ep（上皮型）。通过荧光活化细胞分选分析和酪氨酸酶实验，我们发现只有SK-N-SH-EP和SK-N-SH-F与HMSA-2反应并具有酪氨酸酶活性。这些结果表明上皮型和成纤维型细胞似乎具有黑素细胞的潜能，但不具有神经母细胞的潜能。此外，SK-N-SH-EP被发现可以自发转化为神经母细胞型或成纤维细胞型细胞，而SK-N-SH-N和SK-N-SH-F克隆在形态上保持稳定。我们的研究结果表明，至少有一种神经母细胞瘤细胞系SK-N-SH可能是研究神经母细胞瘤克隆成熟和黑素细胞分化的良好模型。
Five human neuroblastoma cell lines were examined for expression of a human melanosome-associated antigen (HMSA). Only cell line SK-N-SH reacted with a monoclonal antibody, HMSA-2, shown to recognize melanosomal glycoproteins. To further characterize the melanocytic lineages of SK-N-SH, three morphologically distinct clones designated SK-N-SH-N (neuroblast type), SK-N-SH-F (fibroblast type), and SK-N-SH-EP (epithelial type) were established by colony formation cloning. By fluorescence-activated cell sorter analysis and tyrosinase assay, we found that only SK-N-SH-EP and SK-N-SH-F reacted with HMSA-2 and had tyrosinase activity. These results suggest that epithelial-type and fibroblast-type cells appear to possess the melanocytic potential, but not neuroblast-type cells. Furthermore, SK-N-SH-EP was found to spontaneously convert to neuroblast-type or fibroblast-type cells, whereas SK-N-SH-N and SK-N-SH-F clones have remained morphologically stable. Our results suggest that at least one neuroblastoma cell line, SK-N-SH, may be an excellent model for investigating clonal maturation and the melanocytic differentiation of neuroblastoma.