Galectin 3-β-galactobiose interactions
Galectin 3-β-galactobiose interactions
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DOI：
10.1016/j.carbpol.2012.08.104
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发表时间：
2013-01-30
影响因子：
11.2
通讯作者：
Morris, V. J.
中科院分区：
文献类型：
--
作者：
Gunning, A. P.;Pin, C.;Morris, V. J.
力谱已被用于研究二糖β-半乳糖二糖和促转移调节蛋白半乳糖凝集素-3（Gal 3）之间的相互作用。研究揭示了特定的相互作用，其特征在于解离速率解离常数k（off）= 0.33 s（-1），在零作用力下相互作用距离x=0.2 nm。这些数据表明相互作用的寿命为3.0 s。结果与口服食用改性柑橘果胶通过抑制Gal 3的作用来控制癌症转移的假设一致。该修饰被认为有助于果胶衍生的半乳聚糖侧链与Gal 3的结合，并抑制Gal 3作为促转移调节蛋白的作用。(C)2012爱思唯尔有限公司保留所有权利。
Force spectroscopy has been used to investigate the interaction between the disaccharide beta-galactobiose and the pro-metastatic regulatory protein galectin-3 (Gal3). The studies revealed specific interactions characterised by an off-rate dissociation constant k(off) = 0.33 s(-1) and interaction distance x=0.2 nm at zero applied force. These data suggest a lifetime for the interaction of 3.0 s. The results are consistent with the hypothesis that oral consumption of modified citrus pectin controls cancer metastasis by inhibiting the role of Gal3. The modification is considered to facilitate binding of pectin-derived galactan sidechains to Gal3 and inhibition of the roles of Gal3 as a pro-metastatic regulatory protein. (C) 2012 Elsevier Ltd. All rights reserved.