POLYPLOID CELL REPRODUCTION IN NORMAL ADULT RAT LIVER
POLYPLOID CELL REPRODUCTION IN NORMAL ADULT RAT LIVER
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DOI：
10.1016/0014-4827(67)90379-5
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发表时间：
1967-01-01
影响因子：
3.7
通讯作者：
CARRIERE, R
中科院分区：
文献类型：
--
作者：
CARRIERE, R
在 230 g 雄性大鼠 2 小时和 28 小时处死的肝脏的放射自显影制剂中研究肝细胞核。注射氚化胸苷后。注射后两小时，标记的细胞核具有多倍体大小，其中大多数在八倍体大小范围内。注射后二十八小时，标记的细胞核主要在四倍体大小范围内，少数在二倍体和八倍体范围内。其分布与总细胞群中细胞核的分布相同。标记指数为1.0±0.1%，2小时。注射后，显着增加，28小时达到1.9±0.39%。注射后，有丝分裂指数分别为细胞的0.05±0.03%和0.03±0.001%。四倍体大小的细胞核构成了生殖细胞群的很大一部分。他们掺入氚化胸苷并放大至八倍体大小，随后分裂产生子代四倍体细胞核。新的双核细胞是通过新的DNA合成过程形成的，双核细胞正在参与DNA合成。一些细胞核可能通过双核细胞的形成从二倍体状态转变为四倍体状态。
Hepatic cell nuclei were studied in radioautographic preparations of livers from 230 g male rats killed 2 and 28 hr. after injection of tritiated thymidine. Two hours after injection, labelled nuclei were of polyploid size, with a majority in the octoploid size range. Twenty-eight hours after injection, labelled nuclei were predominantly in the tetraploid-size range, with a few in the diploid and octoploid ranges. The distribution was the same as that of nuclei in the total cell population. The labelling index was 1.0 [plus or minus] 0.1%, 2 hr. after injection, and increased significantly, to 1.9 [plus or minus] 0.39%, 28 hr. after injection, while the mitotic indices were 0.05 [plus or minus] 0.03% and 0.03 [plus or minus] 0.001% of cells, respectively. Tetraploid-sized nuclei formed a large proportion of the reproductive cell population. They incorporated tritiated-thymidine and enlarged to octoploid size, subsequently dividing to produce daughter tetraploid nuclei. New binucleated cells were formed through a process involving new DNA synthesis, and binucleated cells were engaging in DNA synthesis. A few nuclei may be shifting from the diploid to the tetraploid state through the formation of binucleated cells.