Determine the position of nanoparticles in cells by using surface-enhanced Raman three-dimensional imaging
Determine the position of nanoparticles in cells by using surface-enhanced Raman three-dimensional imaging
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DOI：
10.1007/s12274-021-3726-z
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发表时间：
2021-07
期刊：
影响因子：
9.9
通讯作者：
Wei Liu;Wentao Li;Yahui Li;Junfang Li;H. Bai;M. Zou;Guangcheng Xi
中科院分区：
文献类型：
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作者：
Wei Liu;Wentao Li;Yahui Li;Junfang Li;H. Bai;M. Zou;Guangcheng Xi
结构，特别是跟踪和监测细胞吞噬纳米颗粒的过程。在此，我们制备了在可见光范围内具有强表面等离子体共振效应的MoO2空心纳米球，表现出良好的表面增强拉曼散射效应。当4-巯基苯甲酸(4-MBA)分子被修饰后，它可以有效地作为拉曼探针分子进行清晰的三维细胞成像。无论纳米粒子位于细胞内、细胞外还是部分细胞内，都可以通过这种增强的拉曼探针分子清晰地呈现出来。这些结果为细胞的三维成像，特别是追踪纳米颗粒的吞噬作用提供了一种快速、准确的方法。
Structure, especially to track and monitor the phagocytosis process of nanoparticles by cells. Herein, we prepared a MoO2hollow nanosphere with a strong surface plasmon resonance effect in the visible light region, which exhibited an excellent surface enhanced Raman scattering effect. When the 4-mercaptobenzoic acid (4-MBA) molecules are modified, it can be efficiently used as Raman probe molecules to perform clear three-dimensional cell imaging. No matter when the nanoparticles are located inside the cell, outside the cell or partly inside the cell, they all can be clearly presented by this enhanced Raman probe molecule. These results provide a rapid and accurate method for three-dimensional imaging of cells, especially for tracking the phagocytosis of nanoparticles.