Her-2 Expression in Gastroesophageal Intestinal Metaplasia, Dysplasia, and Adenocarcinoma.
Her-2 Expression in Gastroesophageal Intestinal Metaplasia, Dysplasia, and Adenocarcinoma.
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DOI：
10.1097/pai.0000000000000243
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发表时间：
2016-10
期刊：
影响因子：
--
通讯作者：
Coppola D
中科院分区：
文献类型：
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作者：
Almhanna K;Rosa M;Henderson-Jackson E;Jiang K;Shamekh R;Sayegh Z;Malafa MP;Coppola D
Barrett 肿瘤中人表皮生长因子受体 2 蛋白 (Her-2) 的过度表达对于曲妥珠单抗的靶向治疗具有重要意义。在这里，我们研究了 Barrett 腺癌和癌前病变中 Her-2 过度表达的频率。使用回顾性福尔马林固定石蜡包埋的组织样本，包括 25 例正常 (NM) 食管粘膜、50 例无不典型增生的 Barrett 食管 (BE)、49 例低度不典型增生 (LGD)、50 例高度不典型增生 (HGD) 的 BE 和 50 例浸润性腺癌 (ICA)。构建 BE 组织微阵列并通过 Her-2 免疫组织化学 (IHC) 和 Her-2 双原位杂交 (DISH) 进行分析。 Her-2 IHC 表达在 NM 中呈阴性，在 26% 的 BE（IHC 评分：1+）和 24.5% 的 LGD（IHC 评分：1 至 2+）中表达较低。 Her-2 过度表达见于 28% 的 HGD 和 24% 的 ICA（IHC 评分：2 至 3+）。 Her-2 DISH 在 NM 和 BE 中呈阴性，但在 6% LGD、20% HGD 和 18% ICA 中呈阳性。 NM 与 HGD 或 ICA 之间的 Her-2 DISH 阳性率差异有统计学意义（P = 0.02），但 NM 与 LGD 或 HGD 与 ICA 之间的差异则无统计学意义（P = 0.2）。尽管 ICA 中的 Her-2 过度表达结果与之前的报道相似，但 HGD 中 28% 的发现是出乎意料的，并且可能具有临床意义。 6% LGD 中 Her-2 DISH 呈阳性是新颖的，表明 Her-2 在 BE 进展过程中发挥作用。
Overexpression of human epidermal growth factor receptor 2 protein (Her-2) in Barrett neoplasia is significant for targeted therapy with trastuzumab. Here, we studied the frequency of Her-2 overexpression in Barrett adenocarcinoma and precursor lesions. Retrospective formalin-fixed paraffin-embedded tissue samples of 25 normal (NM) esophageal mucosa, 50 Barrett esophagus (BE) without dysplasia, 49 BE with low-grade dysplasia (LGD), 50 BE with high-grade dysplasia (HGD), and 50 invasive adenocarcinoma (ICA) were used. A BE tissue microarray was built and analyzed by Her-2 immunohistochemistry (IHC) and Her-2 dual in situ hybridization (DISH). Her-2 IHC expression was negative in NM and low in 26% of BE (IHC score: 1+) and in 24.5% of LGD (IHC score: 1 to 2+). Her-2 overexpression was seen in 28% of HGD and in 24% of ICA (IHC score: 2 to 3+). Her-2 DISH was negative in NM and BE but positive in 6% of LGD, 20% of HGD, and 18% of ICA. Differences in Her-2 DISH positivity between NM and HGD or ICA were statistically significant (P = 0.02), but those between NM and LGD or HGD and ICA were not (P = 0.2). Although Her-2 overexpression results in ICA were similar to previous reports, the finding of 28% in HGD was unexpected and may have clinical implications. Positive Her-2 DISH in 6% of LGD is novel, suggesting a role of Her-2 during BE progression.