Renal press-mediated transfection method for plasmid DNA and siRNA to the kidney
Renal press-mediated transfection method for plasmid DNA and siRNA to the kidney
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DOI：
10.1016/j.bbrc.2008.04.097
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发表时间：
2008-08-01
影响因子：
3.1
通讯作者：
Hashida, Mitsuru
中科院分区：
文献类型：
--
作者：
Mukai, Hidefumi;Kawakami, Shigeru;Hashida, Mitsuru
基因和寡核苷酸转染肾脏的方法是生物医学研究和肾脏疾病治疗的进步所必需的。在本研究中，我们发现，通过简单的方法，包括静脉注射siRNA或质粒DNA后轻轻按压肾脏（肾按压介导的转染法），可以将siRNA以及质粒DNA转染至肾脏。使用荧光素酶作为报告基因，评估基因表达和沉默特性。质粒DNA高效、广泛地转染至受压肾脏周围，同时将siRNA转染至肾脏内，实现基因表达的显着抑制。此外，作为肾功能指标的血清肌酐和血尿素氮水平在通过该方法转染后没有表现出明显变化。因此，看来可以通过肾压介导的转染方法将质粒DNA和siRNA转染至肾脏而不会出现肾功能障碍。 (C) 2008 Elsevier Inc. 保留所有权利。
Gene and oligonucleotides transfection methods to the kidney are required for the progress of biomedical research and the therapy of renal diseases. In this study, we found that siRNA as well as plasmid DNA can be transfected to the kidney by a simple method including lightly and once pressing the kidney after intravenous injection of siRNA or plasmid DNA (renal press-mediated transfection method). Using luciferase as the reporter, gene expression and silencing properties were evaluated. Plasmid DNA is efficiently and widely transfected to the periphery of the pressed kidney, and also siRNA is transfected into the kidney and significant suppression of gene expression can be achieved. Additively, serum creatinine and blood urea nitrogen levels, that are indices of renal function, exhibited no marked changes after transfection by this method. Therefore, it appears that plasmid DNA and siRNA could be transfected to the kidney without renal dysfunction by renal press-mediated transfection method. (C) 2008 Elsevier Inc. All rights reserved.