Evaluation of various electrode materials for detection of oxidized low-density lipoproteins
Evaluation of various electrode materials for detection of oxidized low-density lipoproteins
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各种电极材料检测氧化低密度脂蛋白的评价
DOI：
10.1166/jbn.2013.1518
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发表时间：
2012
影响因子：
2.9
通讯作者：
et al
中科院分区：
文献类型：
--
作者：
Takeda S;et al
测定各种氧化应激产生的氧化低密度脂蛋白（LDL）可能有助于评估心血管疾病的风险。我们评估了一些电极材料，电化学检测氧化LDL。研究了碳纳米管分散液作为电极材料的性能。使用超离心法从正常人血清中分离天然LDL。用CuSO4处理天然LDL制备氧化LDL。通过将纳米管分散体沉积在金电极上，随后干燥来制造电极。在加入天然LDL或氧化LDL之前和之后监测电极相对于参比电极的电位变化。只有酸处理的碳纳米管能够区分两种LDL制剂，这可能是因为酸处理在纳米管上引入了羧酸基团。
Measurement of oxidized low-density lipoprotein (LDL) generated by oxidative stress of various kinds might be useful for evaluating the risk of cardiovascular disease. We evaluated some electrode materials to detect oxidized LDL electrochemically. Some carbon nanotube dispersions were studied as electrode materials. Native LDL was isolated from normal human serum using ultracentrifugation. Oxidized LDL was prepared by treating the native LDL with CuSO4. Electrodes were fabricated by depositing the nanotube dispersion on a gold electrode, with subsequent drying. The potential change of the electrode against a reference electrode was monitored before and after adding native LDL or oxidized LDL. Only acid-treated carbon nanotubes were able to discriminate both LDL preparations, perhaps because of the carboxylic acid groups introduced on the nanotube by acid treatment.