Analysis and clinical relevance of microparticles from red blood cells
Analysis and clinical relevance of microparticles from red blood cells
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DOI：
10.1097/moh.0b013e32833ec217
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发表时间：
2010-11-01
影响因子：
3.2
通讯作者：
Canellini, Giorgia
中科院分区：
文献类型：
--
作者：
Tissot, Jean-Daniel;Rubin, Olivier;Canellini, Giorgia
综述了红细胞微颗粒在体内和红细胞储存过程中形成的机制，并描述了微颗粒在输血医学中的潜在作用。微颗粒的释放是红细胞老化过程中不可分割的一部分，阻止了红细胞的早期清除。蛋白质组学分析概述了3-锚蛋白锚定复合物的关键作用和选择性红细胞膜重塑机制在微颗粒形成中的发生。已证实存在几种红细胞抗原，表达在微粒上。红细胞微粒对输血受者的潜在有害影响，包括高凝性、微循环损伤和免疫抑制，进行了讨论。红细胞微粒的形成和作用还远未完全了解。结合多种途径阐明这些机制可以提高血液制品质量和输血安全。实现红细胞微粒作为生物标志物在实验室常规需要克服技术障碍涉及到他们的分析。
Purpose of reviewThe mechanisms involved in the formation of red blood cell (RBC) microparticles in vivo as well as during erythrocyte storage are reviewed, and the potential role of microparticles in transfusion medicine is described.Recent findingsMicroparticles release is an integral part of the erythrocyte ageing process, preventing early removal of RBCs. Proteomics analyses have outlined the key role of band 3-ankyrin anchoring complex and the occurrence of selective RBC membrane remodelling mechanisms in microparticles formation. The presence of several RBC antigens, expressed on microparticles, has been demonstrated. The potential deleterious effects of RBC microparticles in transfused recipients, including hypercoagulability, microcirculation impairment and immunosuppression, are discussed.SummaryFormation and role of RBC microparticles are far from being completely understood. Combining various approaches to elucidate these mechanisms could improve blood product quality and transfusion safety. Implementation of RBC microparticles as biomarkers in the laboratory routine needs to overcome technical barriers involved in their analysis.