Physical rehabilitation improves muscle function following volumetric muscle loss injury.
Physical rehabilitation improves muscle function following volumetric muscle loss injury.
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DOI：
10.1186/2052-1847-6-41
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发表时间：
2014
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影响因子：
--
通讯作者：
Walters TJ
中科院分区：
文献类型：
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作者：
Aurora A;Garg K;Corona BT;Walters TJ
鉴于临床实践中规定的物理康复治疗VML损伤，本研究探讨了功能和组织形态学的适应体积肌肉损失（VML）受伤的肌肉物理康复。刘易斯大鼠（n = 32）建立胫骨前肌VML损伤模型，随机分为安静（SED）组和物理康复（RUN）组。1周后，RUN大鼠在1周或7周（损伤后2周或8周）内无限制地接触自愿跑步轮。在损伤后2周，收获TA肌肉用于分子分析。在损伤后8周，对大鼠进行体内功能测试。使用组织学和免疫荧光程序分析了组织切片。该研究的主要发现是，自愿轮跑形式的身体康复促进了最大等长扭矩的~ 17%改善，以及受伤肌肉重量的~ 13%增加，但它没有显著的形态适应（例如，无肥大和增生）。车轮运行仅在未受伤的肌肉中上调代谢基因（SIRT-1，PGC-1α），并且在促纤维化基因（胶原蛋白I，TGF-β1）上调之前，在受伤肌肉的缺损区域中有更多的纤维组织沉积。因此，车轮运行相关功能改善是由于力传递改善而不是肌肉再生，这是合理的。这是第一项研究，以证明改善非修复VML受伤肌肉的功能表现与身体康复的形式自愿轮运行。这项研究首次提供了关于VML损伤肌肉在物理康复中的基本变化的信息，这可能有助于制定适当的物理康复方案。
Given the clinical practice of prescribing physical rehabilitation for the treatment of VML injuries, the present study examined the functional and histomorphological adaptations in the volumetric muscle loss (VML) injured muscle to physical rehabilitation. Tibialis anterior muscle VML injury was created in Lewis rats (n = 32), and were randomly assigned to either sedentary (SED) or physical rehabilitation (RUN) group. After 1 week, RUN rats were given unlimited access to voluntary running wheels either 1 or 7 weeks (2 or 8 weeks post-injury). At 2 weeks post-injury, TA muscles were harvested for molecular analyses. At 8 weeks post-injury, the rats underwent in vivo function testing. The explanted tissue was analyzed using histological and immunofluorescence procedures. The primary findings of the study are that physical rehabilitation in the form of voluntary wheel running promotes ~ 17% improvement in maximal isometric torque, and a ~ 13% increase in weight of the injured muscle, but it did so without significant morphological adaptations (e.g., no hypertrophy and hyperplasia). Wheel running up-regulated metabolic genes (SIRT-1, PGC-1α) only in the uninjured muscles, and a greater deposition of fibrous tissue in the defect area of the injured muscle preceded by an up-regulation of pro-fibrotic genes (Collagen I, TGF-β1). Therefore, it is plausible that the wheel running related functional improvements were due to improved force transmission and not muscle regeneration. This is the first study to demonstrate improvement in functional performance of non-repaired VML injured muscle with physical rehabilitation in the form of voluntary wheel running. This study provides information for the first time on the basic changes in the VML injured muscle with physical rehabilitation, which may aid in the development of appropriate physical rehabilitation regimen(s).