GROWTH-RELATED PRODUCTION OF PROTEOGLYCANS AND HYALURONIC-ACID IN SYNCHRONOUS ARTERIAL SMOOTH-MUSCLE CELLS
GROWTH-RELATED PRODUCTION OF PROTEOGLYCANS AND HYALURONIC-ACID IN SYNCHRONOUS ARTERIAL SMOOTH-MUSCLE CELLS
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DOI：
10.1016/0020-711x(92)90040-8
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发表时间：
1992-03-01
期刊：
影响因子：
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通讯作者：
PICARD, J
中科院分区：
文献类型：
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作者：
DEUDON, E;BERROU, E;PICARD, J
1. 研究了血清对动脉平滑肌细胞中蛋白多糖和透明质酸生成的促生长作用。刺激后，与静止细胞相比，[S-35]硫酸盐蛋白聚糖和[C-14]透明质酸的合成在G1和G2期增加（在培养基中分别约为2倍和5倍）。这些蛋白聚糖的大小、电荷和[S-35]糖胺聚糖的相对比例都没有改变。然而，当细胞被刺激分裂时，观察到大[C-14]透明质酸的合成增加，同时产生更高的水动力学尺寸[S-35]蛋白聚糖，这些蛋白聚糖与透明质酸聚集在一起（20%）。
1. The growth-stimulating effect of serum on the proteoglycan and hyaluronic acid production in arterial smooth muscle cells was investigated, using cells synchronized by serum deprivation.2. After stimulation, synthesis of [S-35]sulfated proteoglycans and [C-14]hyaluronic acid increased during G1 and G2 phases (about 2- and 5-fold, respectively, in the culture medium), in comparison with quiescent cells.3. Neither the size, nor the charge, nor the relative proportions of [S-35]glycosaminoglycans of the proteoglycans were modified.4. However, when the cells were stimulated to divide, increased synthesis of large [C-14]hyaluronic acid was observed concomitantly with the production of higher hydrodynamic size [S-35]proteoglycans, which aggregated with hyaluronic acid (20%).