Stimulation of N-methyl-D-aspartate (NMDA) receptors inhibits neuronal migration in embryonic cerebral cortex:: a tissue culture study
Stimulation of N-methyl-D-aspartate (NMDA) receptors inhibits neuronal migration in embryonic cerebral cortex:: a tissue culture study
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DOI：
10.1016/s0165-3806(02)00490-x
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发表时间：
2002-10-20
期刊：
影响因子：
--
通讯作者：
Sawada, T
中科院分区：
文献类型：
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作者：
Kihara, M;Yoshioka, H;Sawada, T
我们利用胚胎大鼠大脑进行了组织培养研究，以研究过度刺激 NMDA 受体对大脑皮层神经元迁移的影响。用[H-3]胸苷标记E16大脑皮层外植体脑室区的祖细胞后，将外植体培养48小时，并通过放射自显影评估标记细胞的分布。在单独的研究中添加到培养基中的 NMDA 和鹅膏酯（NMDA 受体激动剂）对 NMDA 受体的刺激导致中间区的标记细胞百分比显着减少，而心室区的标记细胞百分比增加。结果表明，在大鼠大脑皮层中，NMDA 受体的激动剂刺激会抑制神经元迁移。 (C) 2002 Elsevier Science B.V. 保留所有权利。
We performed a tissue culture study using embryonic rat brain to investigate the effect of excessive stimulation of NMDA receptors on neuronal migration in the cerebral cortex. After progenitor cells in the ventricular zone of E16 cerebral cortex explants were labeled with [H-3]thymidine, the explants were cultured for 48 h, and distributions of labelled cells were evaluated autoradiographically. Stimulation of NMDA receptors by NMDA and ibotenate, NMDA receptor agonists added to the culture medium in separate studies, caused percentages of labeled cells to decrease significantly in the intermediate zone and increase in the ventricular zone. The results suggest that in rat cerebral cortex, agonist stimulation of NMDA receptors inhibits neuronal migration. (C) 2002 Elsevier Science B.V. All rights reserved.