Cellular structural biology as revealed by cryo-electron tomography
Cellular structural biology as revealed by cryo-electron tomography
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DOI：
10.1242/jcs.171967
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发表时间：
2016-02-01
影响因子：
4
通讯作者：
Medalia, Ohad
中科院分区：
文献类型：
--
作者：
Irobalieva, Rossitza N.;Martins, Bruno;Medalia, Ohad
理解细胞机器的功能需要对强调其功能的结构元素进行彻底的分析。电子显微镜（EM）在提供有关细胞超微结构和大分子组织的信息方面起着关键作用。生物材料可以通过玻璃化进行物理固定，并在接近天然条件下用冷冻电子断层扫描（cryo-ET）成像。使用这种技术，人们可以获得有关细胞大分子结构的三维（3D）信息，描绘独特的细胞状态并重建分子网络。过去几年的技术进步，如改进的样品制备和电子检测方法，有助于获得具有前所未有的结构细节的数据。这提供了一个令人兴奋的机会，以纳米到亚纳米分辨率探索单个细胞和多细胞生物体的分子结构。在这篇评论中，我们集中在最近的发展和原位应用冷冻ET细胞和结构生物学。
Understanding the function of cellular machines requires a thorough analysis of the structural elements that underline their function. Electron microscopy (EM) has been pivotal in providing information about cellular ultrastructure, as well as macromolecular organization. Biological materials can be physically fixed by vitrification and imaged with cryo-electron tomography (cryo-ET) in a close-to-native condition. Using this technique, one can acquire three-dimensional (3D) information about the macromolecular architecture of cells, depict unique cellular states and reconstruct molecular networks. Technical advances over the last few years, such as improved sample preparation and electron detection methods, have been instrumental in obtaining data with unprecedented structural details. This presents an exciting opportunity to explore the molecular architecture of both individual cells and multicellular organisms at nanometer to subnanometer resolution. In this Commentary, we focus on the recent developments and in situ applications of cryo-ET to cell and structural biology.