Structural and reproductive modifications in rats following a post-implantational exposure to captan.
Structural and reproductive modifications in rats following a post-implantational exposure to captan.
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植入后接触克菌丹后大鼠的结构和生殖改变。
DOI：
10.1007/bf01625515
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发表时间：
1984
影响因子：
2.7
通讯作者：
Spencer,F
中科院分区：
文献类型：
--
作者：
Spencer,F
材料与方法实验选用雌性Sprague-Dawley大鼠，购自WI. H . tzman公司。实验开始时，这些动物的体重在200-260 g之间，饲养在受控的光周期（光照14小时：黑暗10小时）和温度（20+ 2）的环境条件下，免费提供Purina实验室食物和自来水。显微镜下阴道灌洗法测定发情周期，随机选取连续两次出现4 ~ 5 d发情周期的大鼠进行实验。DCR假妊娠的诱导，如先前所述（Tat-Sing and Spencer 1981），是在发情期和发情期通过刺激宫颈阴道来实现的。随后在刺激后白细胞浸润期的第4天子宫刮伤。怀孕是通过与一个有生育能力的，活跃的男性交配，并通过显微镜观察精子阳性阴道涂片证实。以牛血清白蛋白为标准品，参照Lowry et al.（1951）的方法，用福林酚试剂分析上清蛋白浓度中的可溶性蛋白部分。糖原浓度测定采用Seifter et al.（1950）和Zarrow et al.（1964）改进的方法，以III型兔肝糖原为标准。植入部位通过尾静脉注射芝加哥蓝染料评估，同时剖腹手术和观察子宫角（Labhsetwar 1971）。
MATERIALS AND METHODSThe experiment employed female Sprague-Dawley rats which were purchased from the H oltzman Company, WI. These animals ranged from 200-260 g at the beginning of each experiment and were maintained under controlled ambient conditions of photoperiodicity (14hr light: 10 hr darkness) and temperature (20+ 2~ Purina lab chow and tap water were provided freely. Stages of the estrous cycle were determined microscopically by vaginal lavage, and only those rats which exhibited two consecutive 4 to 5 days estrous cycles were randomly chosen for experimentation. The induction of DCR pseudopregnancy, previously described (Tat-Sing and Spencer 1981), was accomplished by cervicovaginal stimulation during p roestrus and estrus. This was followed by uterine scratch trauma on Day 4 of the period of leucocytic infiltration which followed stimulation. Pregnancy was achieved by mating with a fertile, active male, and verified by the microscopic observation of a spermpositive vaginal smear.The soluble protein fractions of the supernatant protein concentrations were analyzed by the Folin phenol reagent in accordance with the method of Lowry et al.(1951), using bovine serum albumin as the standard. Glycogen concentrations were determined by the method of Seifter et al.(1950) and modified by Zarrow et al.(1964), using Type III rabbit liver glycogen as the standard. Implantation sites were assessed by a tail-vein injection of Chicago blue dye in association with laparotomy and observation of the uterine horns (Labhsetwar 1971).
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ATP 和磷酸盐对重构为磷脂囊泡的 Na-K-ATP 酶介导的铷交换的联合作用
DOI：
--
发表时间：
1982
期刊：
Journal of Physiology
影响因子：
--
作者：
S. Karlish;W. R. Lieb;W. Stein
通讯作者：
W. Stein
哇巴因与 (Na + K)-ATP 酶结合。
DOI：
--
发表时间：
1974
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
T. Tobin;T. Akera;C. Lee;T. Brody
通讯作者：
T. Brody
碳二亚胺使 Na,K-ATP 酶失活。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Pedemonte,CH;Kaplan,JH
通讯作者：
Kaplan,JH
强心类固醇与 Na,K-ATP 酶结合
DOI：
10.1016/s0070-2161(08)60568-7
发表时间：
1983
期刊：
Current topics in membranes and transport
影响因子：
--
作者：
B. Forbush
通讯作者：
B. Forbush
DOI：
10.1016/s0021-9258(17)42732-3
发表时间：
1984-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Farley;C. M. Tran;C. Carilli;D. Hawke;J. Shively
通讯作者：
R. Farley;C. M. Tran;C. Carilli;D. Hawke;J. Shively