STUDIES ON THE DNA ELONGATION INHIBITOR AND ITS PROLIFERATING CELL NUCLEAR ANTIGEN-DEPENDENT CONTROL IN SIMIAN VIRUS-40 DNA-REPLICATION INVITRO
STUDIES ON THE DNA ELONGATION INHIBITOR AND ITS PROLIFERATING CELL NUCLEAR ANTIGEN-DEPENDENT CONTROL IN SIMIAN VIRUS-40 DNA-REPLICATION INVITRO
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DOI：
10.1073/pnas.86.13.4877
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发表时间：
1989-07-01
影响因子：
11.1
通讯作者：
HURWITZ, J
中科院分区：
文献类型：
--
作者：
LEE, SH;KWONG, AD;HURWITZ, J
从HeLa细胞提取液中纯化出一种120 kDa的阻断DNA末端的蛋白质。这种蛋白质抑制了一些酶的作用，这些酶催化涉及DNA 5‘’和3‘’末端的反应(DNA连接酶、3‘’和5‘’核酸外切酶以及DNA聚合酶α)。120 kDa的蛋白质阻止了通常在猿猴病毒40DNA复制过程中形成的长DNA链的合成，导致小DNA片段的积累。这种蛋白质的作用可以通过添加增殖细胞核抗原和其他蛋白质部分(激活剂)来逆转。
A 120-kDa protein that blocks DNA termini has been purified from extracts of HeLa cells. This protein inhibits the action of a number of enzymes that catalyze reactions involving the 5'' and 3'' ends of DNA (DNA ligase, 3'' and 5'' exonucleases, and DNA polymerase .alpha.). The 120-kDa protein blocks the synthesis of long DNA chains that are normally formed during simian virus 40 DNA replication, causing the accumulation of small DNA fragments. The effects of this protein can be reversed by the addition of proliferating cell nuclear antigen and other protein frctions (activators).