Anatomic Study of the Central Core of the Cerebrum Correlating 7-T Magnetic Resonance Imaging and Fiber Dissection With the Aid of a Neuronavigation System
Anatomic Study of the Central Core of the Cerebrum Correlating 7-T Magnetic Resonance Imaging and Fiber Dissection With the Aid of a Neuronavigation System
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DOI：
10.1227/neu.0000000000000271
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发表时间：
2014-06-01
影响因子：
2.3
通讯作者：
Prats-Galino, Alberto
中科院分区：
文献类型：
--
作者：
Alarcon, Carlos;de Notaris, Matteo;Prats-Galino, Alberto
背景：在体内和离体研究人脑纤维束解剖的方法不同。然而，研究白质解剖的理想方法尚未确定，因为它应该整合从多个来源获得的信息。目的：我们建立了一种解剖学方法，将传统的白质解剖与使用神经导航系统共登记的同一标本的高分辨率7-T磁共振成像（MRI）相结合，研究大脑中央核心。方法：采用传统的Klingler技术制备10个大脑半球。在解剖前，对每个半球进行结构超高磁场7-T MRI研究，这些研究特别制备了用于神经导航的表面基准。然后使用经典的白色纤维剥离技术从内侧半球表面进行剥离。在解剖的每个步骤中，用神经导航系统评估解剖结果与7-T MRI之间的相关性。结果：解剖研究分为间脑和边缘两个阶段。间脑期包括上皮、丘脑、下丘脑和下丘脑成分。边缘阶段包括扩展解剖以完成帕佩兹回路。两种方法结合所提供的详细信息使我们能够识别和验证可能难以识别和解剖的纤维的位置（即内侧前脑束）。结论：高分辨率7-T MRI与神经导航白质剥离技术的相关性，显著提高了对人类大脑复杂区域结构连接的认识。
BACKGROUND: Different strategies have been used to study the fiber tract anatomy of the human brain in vivo and ex vivo. Nevertheless, the ideal method to study white matter anatomy has yet to be determined because it should integrate information obtained from multiple sources.OBJECTIVE: We developed an anatomic method in cadaveric specimens to study the central core of the cerebrum combining traditional white matter dissection with high-resolution 7-T magnetic resonance imaging (MRI) of the same specimen coregistered using a neuronavigation system.METHODS: Ten cerebral hemispheres were prepared using the traditional Klingler technique. Before dissection, a structural ultrahigh magnetic field 7-T MRI study was performed on each hemisphere specifically prepared with surface fiducials for neuronavigation. The dissection was then performed from the medial hemispheric surface using the classic white fiber dissection technique. During each step of the dissection, the correlation between the anatomic findings and the 7-T MRI was evaluated with the neuronavigation system.RESULTS: The anatomic study was divided in 2 stages: diencephalic and limbic. The diencephalic stage included epithalamic, thalamic, hypothalamic, and subthalamic components. The limbic stage consisted of extending the dissection to complete the Papez circuit. The detailed information given by the combination of both methods allowed us to identify and validate the position of fibers that may be difficult to appreciate and dissect (ie, the medial forebrain bundle).CONCLUSION: The correlation of high-definition 7-T MRI and the white matter dissection technique with neuronavigation significantly improves the understanding of the structural connections in complex areas of the human cerebrum.