Human renal carcinoma cells produce hypercalcemia in the nude mouse and a novel protein recognized by parathyroid hormone receptors.
Human renal carcinoma cells produce hypercalcemia in the nude mouse and a novel protein recognized by parathyroid hormone receptors.
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DOI：
10.1172/jci110825
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发表时间：
1983-03
期刊：
影响因子：
--
通讯作者：
G. Strewler;R. Williams;R. Nissenson
中科院分区：
文献类型：
--
作者：
G. Strewler;R. Williams;R. Nissenson
当在裸鼠中生长时，来自高钙血症患者的培养的肾癌细胞产生显著的高钙血症，其通过肿瘤切除而逆转。来自该细胞系的条件培养基含有在肾腺苷酸环化酶生物测定中对甲状旁腺激素（PTH）具有活性的蛋白质，其被竞争性PTH拮抗剂[8正亮氨酰，18正亮氨酰，34酪氨酸酰]bPTH（3-34）酰胺阻断。然而，生物活性蛋白作为分子大小比PTH更大的组分从凝胶过滤柱中洗脱，并且不被四种区域特异性PTH抗血清中的任何一种识别。该因子的性质类似于恶性肿瘤体液性高钙血症中假定的PTH样物质。
When grown in nude mice, cultured renal carcinoma cells from a hypercalcemic patient produced marked hypercalcemia that was reversed by resection of tumor. Conditioned medium from this cell line contained a protein with activity in a renal adenylate cyclase bioassay for parathyroid hormone (PTH) which was blocked by the competitive PTH antagonist [8norleucyl, 18norleucyl, 34tyrosinyl]bPTH (3-34)amide. However, the biologically active protein was eluted from gel filtration columns as a larger molecular size component that PTH and was not recognized by any of four region-specific PTH antisera. The properties of this factor resemble those of the postulated PTH-like substance(s) in humoral hypercalcemia of malignancy.