MagT1 is essential for Drosophila development through the shaping of Wingless and Decapentaplegic signaling pathways
MagT1 is essential for Drosophila development through the shaping of Wingless and Decapentaplegic signaling pathways
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MagT1 通过塑造 Wingless 和 Decapentaplegic 信号通路对于果蝇发育至关重要
DOI：
10.1016/j.bbrc.2018.06.133
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发表时间：
2018
影响因子：
3.1
通讯作者：
Fang Ming
中科院分区：
文献类型：
--
作者：
Xun Qingying;Bi Caili;Cui Xiaoying;Wu Hongyan;Wang Mingying;Liao Yanlin;Wang Rui;Xie Hao;Shen Zhijun;Fang Ming
镁转运子亚型1（MagT 1）是具有通道样性质的镁膜转运子。我们以前在果蝇基因组中鉴定了MagT 1（CG 7830），并通过电生理手段表征了其蛋白产物。在这里，我们报告了flyMagT 1突变体的产生，并表明MagT 1是早期胚胎发育所必需的。在翅膀和原始翅膀中，通过克隆分析和RNAi敲除MagT 1，我们发现MagT 1的缺失导致增强/异位Wingless（Wg，一种苍蝇Wnt）信号和破坏Decapentaplegic（Dpp）信号，表明MagT 1在苍蝇后期发育中的关键作用。最后，我们直接证明了果蝇S2细胞中镁的转运与MagT 1的表达水平成正比。综上所述，这些发现可能表明，MagT 1是一个主要的镁转运蛋白/通道，通过影响发育信号通路，如Wg和Dpp信号，深刻参与苍蝇的发展。
Magnesium transporter subtype 1 (MagT1) is a magnesium membrane transporter with channel like properties. We have previously identifiedMagT1(CG7830) inDrosophilagenome and characterized its protein product by electrophysiological means. Here, we report the generation of flyMagT1mutants and show thatMagT1is essential for early embryonic development. In wings and primordial wings, by clonal analysis and RNAi knock down ofMagT1, we have found that loss ofMagT1results in enhanced/ectopic Wingless (Wg, a fly Wnt) signaling and disrupted Decapentaplegic (Dpp) signaling, indicating the crucial role ofMagT1for fly development at later stages. Finally, we demonstrate directly that magnesium transportations are proportional with theMagT1expressional levels inDrosophilaS2 cells. Taken together, these findings may suggest that MagT1 is a major magnesium transporter/channel profoundly involved in fly development by affecting developmental signaling pathways, such as Wg and Dpp signaling.