Investigation of chemical linkage between lignin and carbohydrates in cultured poplar cambium tissues with double isotope labeling
Investigation of chemical linkage between lignin and carbohydrates in cultured poplar cambium tissues with double isotope labeling
复制标题
双同位素标记研究培养杨树形成层组织中木质素和碳水化合物之间的化学键
DOI：
10.1016/j.ijbiomac.2023.123250
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发表时间：
2023
影响因子：
8.2
通讯作者：
Yimin Xie
中科院分区：
文献类型：
--
作者：
Sheng Cui;Xin Wei;Xudong Chen;Yimin Xie
Lignin precursor labeled with 13C (coniferin-13Cα), carbohydrate precursor labeled with D (6,6-D2-glucose) were .put into cambium tissue stripped from a growing poplar. The tissue was further cultured in vitro for 18d. Then, .the isotopic abundance was determined. The results showed that the labeled precursors could be normally .involved in the formation of new xylem. The labeled new xylem tissue was fractionated by ionic liquid DMSO/ .TBAH system to obtain two components: glucan-lignin complex (GL) and xylan-lignin complex (XL). The X-ray .diffraction (XRD) results indicated that the crystalline form of cellulose in the GL component was transformed .from type I to type II after the ionic liquid separation. Then the GL and XL were purified and modified by .enzymatic and chemical methods, and their structures were elucidated by nuclear magnetic resonance (NMR) .spectroscopy. The results showed that lignin subunits in the cultured tissues were mainly connected by β-5 and .β-O-4 linkages, of which the β-O-4 substructure unit predominated. Lignin and carbohydrates were mainly .connected by acetal bonds, ether bonds, and ester bonds. Combined with the carbohydrate composition and XRD .analysis results, the GL components also confirmed the existence of acetal bonds, ester bonds and ether bonds .between lignin and cellulose.
