A wireless point-of-care testing system for the detection of neuron-specific enolase with microfluidic paper-based analytical devices
A wireless point-of-care testing system for the detection of neuron-specific enolase with microfluidic paper-based analytical devices
复制标题
无线即时检测系统，用于使用微流体纸基分析设备检测神经元特异性烯醇化酶
DOI：
10.1016/j.bios.2017.04.003
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发表时间：
2017-09-15
影响因子：
12.6
通讯作者：
Cai, Xinxia
中科院分区：
文献类型：
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作者：
Fan, Yan;Liu, Juntao;Cai, Xinxia
神经元特异性烯醇化酶（NSE）对小细胞肺癌的诊断、分期、监测效果及判断预后具有临床意义。因此，对NSE现场测试的需求不断增长。在这里，开发并验证了一种用于NSE检测的带有电化学测量的无线护理点测试（POCT）系统。无线POCT系统由微流控纸基分析装置（pad）、电化学检测器和Android智能手机组成。差分脉冲伏安法（DPV）测量采用电化学检测器，其中包括一个恒电位器和一个电流-电压转换器。用氨基官能团石墨烯、硫氨酸和金纳米粒子（NH2-G/Thi/AuNPs）合成的纳米复合材料修饰pPADs作为NSE检测的免疫传感器。结合pad对无线POCT系统的性能进行了评估。NSE浓度的对数在1 ~ 500 ng mL（-1）范围内与检测限（LOD）为10 pg mL（-1）呈良好的线性关系。检测结果自动存储在EEPROM存储器中，并可通过蓝牙实时显示在Android智能手机上。检测结果与商用电化学工作站的检测结果相当。无线POCT系统具有现场检测其他肿瘤标志物的潜力。
Neuron-specific enolase (NSE) had clinical significance on diagnosis, staging, monitoring effect and judging prognosis of small cell lung cancer. Thus, there had a growing demand for the on-site testing of NSE. Here, a wireless point-of-care testing (POCT) system with electrochemical measurement for NSE detection was developed and verified. The wireless POCT system consisted of microfluidic paper-based analytical devices (PADs), electrochemical detector and Android's smartphone. Differential pulse voltammetry (DPV) measurement was adopted by means of electrochemical detector which including a potentiostat and current-to-voltage converter. pPADs were modified with nanocomposites synthesized by Amino functional graphene, thionine and gold nanoparticles (NH2-G/Thi/AuNPs) as immunosensors for NSE detection. Combined with PADs, the performance of the wireless POCT system was evaluated. The peak currents showed good linear relationship of the logarithm of NSE concentration ranging from 1 to 500 ng mL(-1) with the limit of detection (LOD) of 10 pg mL(-1). The detection results were automatically stored in EEPROM memory and could be displayed on Android's smartphone through Bluetooth in real time. The detection results were comparable to those measured by a commercial electrochemical workstation. The wireless POCT system had the potential for on-site testing of other tumor markers.