Pyrosequencing analysis of bacterial diversity in soils contaminated long-term with PAHs and heavy metals: Implications to bioremediation
Pyrosequencing analysis of bacterial diversity in soils contaminated long-term with PAHs and heavy metals: Implications to bioremediation
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DOI：
10.1016/j.jhazmat.2016.05.066
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发表时间：
2016-11-05
影响因子：
13.6
通讯作者：
Naidu, Ravi
中科院分区：
文献类型：
--
作者：
Kuppusamy, Saranya;Thavamani, Palanisami;Naidu, Ravi
采用454焦磷酸测序技术，首次研究了多环芳烃（PAHs）和重金属长期污染土壤中细菌群落的多样性、分布和组成。引人注目的是，在我们的研究中获得的革兰氏阳性（aye）和革兰氏阴性（-ve）细菌谱的完整图片说明了新的假设，包括：（1）属于α变形菌纲的耐重金属和降解多环芳烃的革兰氏阳性菌在真实的污染场地中的存活率比革兰氏阳性菌高。（2）革兰氏阳性菌对重金属的毒性并不总是具有抗性，（3）寡养单胞菌属（Stenotrophomonas）、伯克霍尔德菌属（Burkholderia）和假单胞菌属（Pseudomonas）是长期污染土壤中具有较高代谢活性的多环芳烃降解菌的优势菌属。（4）放线菌是高分子量多环芳烃污染土壤中与Pb、Cu、Zn等有毒重金属共存的革兰氏菌属的优势菌群。（5）微生物群落在自然环境中是营养驱动的;（6）革兰氏阳性菌在历史污染土壤中最终进化为具有不同适用性的具有分解代谢潜力的革兰氏阳性菌。因此，需要利用来自长期污染场地的最有前途的本土革兰氏+/-ve菌株进行混合污染物清理，这些菌株具有增加的分解代谢潜力、酶活性和金属耐受性。(C)2016爱思唯尔B. V.保留所有权利。
Diversity, distribution and composition of bacterial community of soils contaminated long-term with both polycyclic aromatic hydrocarbons (PAHs) and heavy metals were explored for the first time following 454 pyrosequencing. Strikingly, the complete picture of the Gram positive (aye) and Gram negative (-ve) bacterial profile obtained in our study illustrates novel postulates that include: (1) Metal-tolerant and PAH-degrading Gram +ves belonging to the class Alphaproteobacteria persist relatively more in the real contaminated sites compared to Gram +ves, (2) Gram ayes are not always resistant to heavy metal toxicity, (3) Stenotrophomonas followed by Burkholderia and Pseudomonas are the dominant genera of PAH degraders with high metabolic activity in long-term contaminated soils, (4) Actinobacteria is the predominant group among the Gram +ves in soils contaminated with high molecular weight PAHs that co-exist with toxic heavy metals like Pb, Cu and Zn, (5) Microbial communities are nutrient-driven in natural environments and (6) Catabolically potential Gram +1 ves with diverse applicability to remediate the real contaminated sites evolve eventually in the historically-polluted soils. Thus, the most promising indigenous Gram +/-ve strains from the long-term contaminated sites with increased catabolic potential, enzymatic activity and metal tolerance need to be harnessed for mixed contaminant cleanups. (C) 2016 Elsevier B.V. All rights reserved.