Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling.
Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling.
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DOI：
10.1038/ncomms7265
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发表时间：
2015-02-13
影响因子：
16.6
通讯作者：
Humphries, Martin J.
中科院分区：
文献类型：
--
作者：
Robertson, Joseph;Jacquemet, Guillaume;Byron, Adam;Jones, Matthew C.;Warwood, Stacey;Selley, Julian N.;Knight, David;Humphries, Jonathan D.;Humphries, Martin J.
细胞-细胞外基质(ECM)的黏附是多细胞存在的基本要求，因为它在定位、增殖和分化中起着重要作用。磷酸化在黏附信号转导中起主要作用；然而，对黏附部位发生的磷酸化事件缺乏充分的了解。在这里，我们报告了从细胞中分离出来的黏附复合体在纤维连接蛋白上的蛋白质组和磷蛋白质组分析。我们鉴定了1,174个蛋白质，其中499个是磷酸化的(1,109个磷酸化位点)，包括特征良好的和新的黏附调节的磷酸化事件。免疫印迹表明，在粘连部位发现了两类磷酸化残基--粘连诱导的残基和因粘连而被成分磷酸化但被募集的残基。激酶预测分析确定了可能在黏附信号转导中发挥作用的新的激酶，包括CDK1，抑制CDK1可以减少黏附复合体的形成。这种磷粘附性的数据集构成了一个宝贵的资源，可以提高我们对细胞-细胞外基质相互作用控制细胞行为的信号机制的理解。蛋白质磷酸化在细胞黏附信号转导中起重要作用。Robertson等人。提供了一个蛋白质组学资源，描绘了从黏附复合体中分离出的蛋白质的磷酸化状态，并利用这个数据集，表明细胞周期激酶CDK1可能影响细胞黏附。
Cell–extracellular matrix (ECM) adhesion is a fundamental requirement for multicellular existence due to roles in positioning, proliferation and differentiation. Phosphorylation plays a major role in adhesion signalling; however, a full understanding of the phosphorylation events that occur at sites of adhesion is lacking. Here we report a proteomic and phosphoproteomic analysis of adhesion complexes isolated from cells spread on fibronectin. We identify 1,174 proteins, 499 of which are phosphorylated (1,109 phosphorylation sites), including both well-characterized and novel adhesion-regulated phosphorylation events. Immunoblotting suggests that two classes of phosphorylated residues are found at adhesion sites—those induced by adhesion and those constitutively phosphorylated but recruited in response to adhesion. Kinase prediction analysis identifies novel kinases with putative roles in adhesion signalling including CDK1, inhibition of which reduces adhesion complex formation. This phospho-adhesome data set constitutes a valuable resource to improve our understanding of the signalling mechanisms through which cell–ECM interactions control cell behaviour. Protein phosphorylation is known to play an important role in cell adhesion signalling. Robertson et al. present a proteomic resource mapping the phosphorylation states of proteins isolated from adhesion complexes and, taking advantage of this data set, show that the cell cycle kinase CDK1 may influence cell adhesion.
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影响因子：
4.4
作者：
Liu, Li;Schwartz, Barbara;Schnapp, Lynn M.
通讯作者：
Schnapp, Lynn M.
影响因子：
7.3
作者：
Humphries JD;Byron A;Bass MD;Craig SE;Pinney JW;Knight D;Humphries MJ
通讯作者：
Humphries MJ
DOI：
10.1083/jcb.200212172
发表时间：
2003-05-26
期刊：
The Journal of cell biology
影响因子：
--
作者：
Manes T;Zheng DQ;Tognin S;Woodard AS;Marchisio PC;Languino LR
通讯作者：
Languino LR
影响因子：
4.1
作者：
Curtis, M;Nikolopoulos, SN;Turner, CE
通讯作者：
Turner, CE
影响因子：
4
作者：
Kirchner, J;Kam, Z;Geiger, B
通讯作者：
Geiger, B