Molecular Threading-Dependent Mass Transport in Paper Origami for Single-Step Electrochemical DNA Sensors.
Molecular Threading-Dependent Mass Transport in Paper Origami for Single-Step Electrochemical DNA Sensors.
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DOI：
10.1021/acs.nanolett.8b04051
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发表时间：
2018-12
期刊：
影响因子：
10.8
通讯作者：
Dekai Ye;Li Li-Li;Zhenhua Li;Yueyue Zhang;Min Li;Jiye Shi;Lihua Wang;C. Fan;Jinghua Yu
中科院分区：
文献类型：
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作者：
Dekai Ye;Li Li-Li;Zhenhua Li;Yueyue Zhang;Min Li;Jiye Shi;Lihua Wang;C. Fan;Jinghua Yu
分子运输控制复杂生物网络系统的效率，例如细胞信号系统和级联生物医学反应。然而，分子传感的设备制造往往受到传输效率低和加工复杂的限制。在这里，我们报告了一种使用三维（3D）纸折纸的分子线依赖性传输系统，可实现生物分子的定向传输。我们证明，基于框架核酸的界面工程允许正交分子识别和现场编程顺序的酶促反应。因此，我们开发了一种单步电化学 DNA 传感器，可在 60 分钟内实现 1 皮摩尔灵敏度的定量分析。我们的传感器可以在单碱基不匹配的水平上区分不匹配的目标。我们的研究显示了开发用于现场护理和精确诊断的仿生分子运输系统的巨大潜力。
Molecular transport controls the efficiency of complex biological network systems such as cellular signaling system and cascade biomedical reaction. However, device fabrication for molecular sensing is often restricted by a low transport efficiency and complicated processing. Here, we report a molecular threading-dependent transport system using three-dimensional (3D) paper origami enabling the directional transport of biomolecules. We demonstrate that framework nucleic acid-based interface engineering allows orthogonal molecular recognition and enzymatic reaction with programmed order on site. We thus develop a single-step electrochemical DNA sensor for quantitative analysis with 1 picomolar sensitivity within 60 min. Our sensor can discriminate a mismatched target at the level of a single base mismatch. Our study shows a great potential toward the development of a biomimetic molecular transport system for point-of-care and precision diagnosis.