Development of mesenchymal stem cells partially originate from the neural crest
Development of mesenchymal stem cells partially originate from the neural crest
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DOI：
10.1016/j.bbrc.2009.01.031
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发表时间：
2009-02-20
影响因子：
3.1
通讯作者：
Matsuzaki, Yumi
中科院分区：
文献类型：
--
作者：
Morikawa, Satoru;Mabuchi, Yo;Matsuzaki, Yumi
间充质干细胞（Mesenchymal stem cells，MSC）是从许多成体组织中分离的一种异质性基质干细胞。先前的研究报道了MSC可以通过称为“去分化”或“转分化”的现象分化为中胚层和神经谱系。然而，由于MSC仅在体外被定义，因此其在体内的大部分发育仍是未知的。在这里，我们前瞻性地确定了骨髓间充质干细胞从成年转基因小鼠编码神经嵴特异性P0-Cre/Floxed-EGFP和Wnt 1-Cre/Floxed-EGFP。EGFP阳性MSCs形成表达神经嵴干细胞基因的球体，并分化为神经元、神经胶质细胞和肌成纤维细胞。有趣的是，我们在GFP（+）和GFP（-）组分中都观察到MSC，并且发现这两个群体之间的体外特征没有显著差异。我们的研究结果表明，成人骨髓中的MSC至少有两个发育起源，其中之一是神经嵴。(C)2009 Elsevier Inc. All rights reserved.
Mesenchymal stem cells (MSCs) are a heterogeneous subset of stromal stem cells isolated from many adult tissues. Previous studies reported that MSCs can differentiate to both mesodermal and neural lineages by a phenomenon referred to as "dedifferentiation" or "transdifferentiation". However, since MSCs have only been defined in vitro, Much of their development in Vivo is Still unknown. Here, we prospectively identified MSCs in the bone marrow from adult transgenic mice encoding neural crest-specific P0-Cre/FIoxed-EGFP and Wnt1-Cre/Floxed-EGFP. EGFP-positive MSCs formed spheres that expressed neural crest stem cell genes and differentiated into neurons, glial cells, and myofibroblasts. Interestingly, we observed MSCs both in the GFP(+) and GFP(-) fraction and found that there were no significant differences in the in vitro characteristics between these two populations. Our results suggest that MSCs in adult bone marrow have at least two developmental origins, one of which is the neural crest. (C) 2009 Elsevier Inc. All rights reserved.