DEVELOPMENT OF A SUBMICROMETER OPTICAL-FIBER OXYGEN SENSOR
DEVELOPMENT OF A SUBMICROMETER OPTICAL-FIBER OXYGEN SENSOR
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DOI：
10.1021/ac00111a024
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发表时间：
1995-08-01
影响因子：
7.4
通讯作者：
KOPELMAN, R
中科院分区：
文献类型：
--
作者：
ROSENZWEIG, Z;KOPELMAN, R
基于三(1，10-邻菲咯啉)Ru(II)在氧气或溶解氧存在下的荧光猝灭，制备了亚微米级光纤氧传感器，将Ru化合物引入丙烯酰胺聚合物中，通过光引发聚合将其共价连接到硅烷化的光纤尖端表面。通过优化丙烯酰胺单体和交联剂N，N-亚甲基双丙烯酰胺之间的比例，使敏感试剂从聚合物基质中的浸出量降至最低。该传感器是完全可逆的，重复性很高。对于几种氧浓度，连续10次荧光测量的标准偏差类似于2%。测量所需的样品体积为100fl。绝对检出限为1×10(-17)摩尔。与其他现有的光纤氧传感器相比，这是一个10(6)倍的改进。
A submicrometer optical fiber oxygen sensor has been fabricated, based on the fluorescence quenching of tris(1,10-phenanthroline)ruthenium(II) chloride in the presence of oxygen or dissolved oxygen, The Ru compound has been incorporated into acrylamide polymer that is attached covalently to a silanized optical fiber tip surface by photoinitiated polymerization. Leaching of the sensing reagent from the polymer host matrix has been minimized by the optimization of the ratio between the acrylamide monomer and the cross-linker, N,N-methylenebisacrylamide. The sensor is fully reversible and highly reproducible. A standard deviation of similar to 2% for 10 consecutive fluorescence measurements has been observed for several oxygen concentrations. The sample volume required for measurements is 100 fL. An absolute detection limit of 1 x 10(-17) mol is achieved. This is an improvement by a factor of 10(6) as compared to other existing optical fiber oxygen sensors.