THE REPERTOIRE OF SILENT PILUS GENES IN NEISSERIA-GONORRHOEAE - EVIDENCE FOR GENE CONVERSION
THE REPERTOIRE OF SILENT PILUS GENES IN NEISSERIA-GONORRHOEAE - EVIDENCE FOR GENE CONVERSION
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DOI：
10.1016/0092-8674(86)90489-7
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发表时间：
1986-01-01
期刊：
影响因子：
64.5
通讯作者：
MEYER, TF
中科院分区：
文献类型：
--
作者：
HAAS, R;MEYER, TF
目的探讨沉默基因位点对菌毛抗原变异的意义。淋病，我们确定了主要沉默基因座，pilS 1的核苷酸序列。pilsi基因座含有6个串联菌毛基因拷贝，其由也存在于表达基因座中的39 bp重复序列连接。所有沉默拷贝都缺乏菌毛蛋白的共同N-末端编码序列，而是含有构成半变（SV）和高变（HV）结构域的变体序列信息。SV和HV结构域散布有短的、严格保守的（C）区域，其侧接可变序列信息的小盒。似乎这种来自沉默拷贝的小片段可以通过基因转换的方式复制并转移到其他沉默或表达基因。
To investigate the significance of silent gene loci for pilus antigenic variation in N. gonorrhoeae, we determined the nucleotide sequence of the major silent locus, pilS1. The pilsi locus contains six tandem pilus gene copies linked by a 39 bp repeat sequence also present in the expression loci. All silent copies lack the common N-terminal coding sequence of pilin, containing instead variant sequence information that constitutes a semivariable (SV) and a hypervariable (HV) domain. The SV and HV domains are interspersed with short, strictly conserved (C) regions flanking small cassettes of variable sequence information. It appears that such minicassettes from silent copies can be duplicated and transferred to other silent or expression genes by means of gene conversion.