Coating of nanoparticles on cryogel surface and subsequent double-modification for enhanced ion-exchange capacity of protein
Coating of nanoparticles on cryogel surface and subsequent double-modification for enhanced ion-exchange capacity of protein
复制标题
在冷冻凝胶表面涂覆纳米颗粒并随后进行双重修饰以增强蛋白质的离子交换能力
DOI：
10.1016/j.chroma.2014.07.019
复制
发表时间：
2014
影响因子：
4.1
通讯作者：
Sun Yan
中科院分区：
文献类型：
--
作者：
Tao Shi-Peng;Wang Chuan;Sun Yan
通过在聚丙烯酰胺（PAM）凝胶孔壁表面包覆聚甲基丙烯酸缩水甘油酯纳米粒子（NPs），制备了一种新型的复合凝胶整体柱。采用聚乙烯亚胺（PEI）和二乙基氨基乙基（二乙胺乙基）对NPs包被柱进行双重修饰。扫描电子显微镜显示纳米粒子在冻凝胶表面的致密涂层，但纳米粒子涂层并没有导致显着的变化的柱的孔隙率和渗透性。粗糙的孔壁表面和延伸的聚合物链提供了更多的结合位点，因此在6 cm/min的流速下，复合晶胶床对牛血清白蛋白的动态结合容量达到11.7 mg/mL床体积，是未包覆纳米粒子的PEI修饰晶胶床（2.8 mg/mL）的4.2倍。结合能力以及柱效率仅略有下降，增加流速从0.6至12厘米/分钟。结果表明，战略的NP涂层结合双离子交换剂的修改是有前途的，以提高冷冻凝胶容量，和新的材料将是有用的高速蛋白质色谱。
A novel composite cryogel monolith was developed by coating poly(glycidyl methacrylate) nanoparticles (NPs) onto the pore wall surface of poly(acrylamide) cryogel. The NPs-coated column was double-modified with poly(ethylenimine) (PEI) and diethylaminoethyl in sequence. Scanning electron microscopy revealed the dense coating of the NPs on the cryogel surface, but the NPs-coating did not result in distinct changes of the column porosity and permeability. The rough pore wall surface and extended polymer chains offered more binding sites, so the dynamic binding capacity of the composite cryogel bed for bovine serum albumin reached 11.7 mg/mL bed volume at a flow rate of 6 cm/min, which was 4.2 times higher than that of the cryogel bed modified with PEI without coating NPs (2.8 mg/mL). The binding capacity as well as column efficiency decreased only slightly with increasing flow rate from 0.6 to 12 cm/min. The results indicated that the strategy of NPs-coating incorporating with double ion-exchanger modifications is promising for enhancing cryogel capacities, and the novel material would be useful for high-speed protein chromatography.