Genome structure of the defective avian sarcoma virus PRCIV.
Genome structure of the defective avian sarcoma virus PRCIV.
复制标题
有缺陷的禽肉瘤病毒 PRCIV 的基因组结构。
DOI：
10.1016/0042-6822(82)90515-3
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发表时间：
1982
期刊：
影响因子：
3.7
通讯作者：
Vogt,PK
中科院分区：
文献类型：
--
作者：
Wong,TC;Hirano,A;Lai,MM;Vogt,PK
应用核酸杂交和寡核苷酸作图技术对禽肉瘤病毒PRCIV的基因组进行了研究。它的长度约为6.1 kb。一段逆转录病毒复制序列缺失，包括部分gag、全部pol和大部分env区，共约5kb。PRCIV特异性取代的两侧是辅助病毒共有的序列，5′端的一段2.0kb的序列，包括部分gag区，3′端的一段1kb的序列，包括C区和可能的一些env序列。制备代表转化特异性序列的PRCIV特异性cDNA探针。该探针仅与其他II类肉瘤病毒的RNA杂交，但不能与I类或III类肉瘤病毒杂交。
The genome of PRCIV, a defective avian sarcoma virus of class II, was studied by nucleic acid hybridization and oligonucleotide mapping. It has a length of about 6.1 kilobases (kb). A stretch of retrovirus replicative sequences encompassing part of thegag, all of thepol, and most of theenvregion, in all about 5 kb, is deleted. In its place a 2.9 kb substitution that is transformation-specific for PRCIV was found. Flanking the PRCIV-specific substitution are sequences shared with the helper virus, a stretch of 2.0 kb on the 5′-side, including part of thegagregion, and a stretch of up to 1 kb on the 3′-side, including the C-region and possibly someenvsequences. A PRCIV-specific cDNA probe representing the transformation-specific sequences was prepared. This probe hybridizes only with RNAs of other class II sarcoma viruses but fails to hybridize with either class I or class III sarcoma viruses.