Splenic red cell sequestration and blood volume measurements in conscious pigs.
Splenic red cell sequestration and blood volume measurements in conscious pigs.
复制标题
清醒猪的脾红细胞隔离和血容量测量。
DOI：
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发表时间：
1985
影响因子：
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通讯作者：
W. Rodkey
中科院分区：
文献类型：
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作者：
J. P. Hannon;C. Bossone;W. Rodkey
在接近基础条件下，通过51cr标记红细胞的稀释估计，未成熟脾切除猪（n = 20）的循环红细胞体积为17.8 +/- 1.64 (SD) ml/kg。假设体大血管红细胞比容（BH:LH）为0.9，血浆容量为49.6 +/- 3.12 ml/kg，血容量为67.3 +/- 3.67 ml/kg。假手术猪20头，循环红细胞体积16.2 +/- 1.39 ml/kg，血浆体积51.1 +/- 3.42 ml/kg，血容量67.2 +/- 4.12 ml/kg。假手术猪循环血液中51Cr早期损失的动力学分析表明，脾脏红细胞隔离为4.5 +/- 0.89 ml/kg，脾脏红细胞周转时间为9.76 +/- 1.93 min。注射肾上腺素（n = 6）和身体约束（n = 8）使假手术猪脾红细胞快速动员。根据同时稀释51cr标记的红细胞和125i标记的牛白蛋白，脾切除猪（n = 7）的体积估计，循环红细胞、血浆和血液体积分别为18.4 +/- 2.46、60.7 +/- 4.01和79.0 +/- 3.51 ml/kg， BH:LH比为0.756 +/- 0.029。后一个值可能反映了125i标记白蛋白程序对血浆容量的高估。
When estimated by the dilution of 51Cr-labeled red blood cells under nearly basal conditions, immature splenectomized pigs (n = 20) had a circulating red cell volume of 17.8 +/- 1.64 (SD) ml/kg. At an assumed body-to-large vessel hematocrit (BH:LH) ratio of 0.9, plasma volume was 49.6 +/- 3.12 ml/kg and blood volume 67.3 +/- 3.67 ml/kg. Sham-operated pigs (n = 20) had a circulating red cell volume of 16.2 +/- 1.39 ml/kg, a plasma volume of 51.1 +/- 3.42 ml/kg, and blood volume of 67.2 +/- 4.12 ml/kg. Kinetic analysis of early 51Cr loss from the circulating blood of the sham-operated pigs indicated a splenic red cell sequestration of 4.5 +/- 0.89 ml/kg and a t1/2 of 9.76 +/- 1.93 min for splenic red cell turnover. Epinephrine injection (n = 6) and physical restraint (n = 8) caused rapid mobilization of splenic red blood cells in sham-operated pigs. Volume estimates in splenectomized pigs (n = 7) based on simultaneous dilutions of 51Cr-labeled red blood cells and 125I-labeled bovine albumin gave circulating red cell, plasma, and blood volumes of 18.4 +/- 2.46, 60.7 +/- 4.01, and 79.0 +/- 3.51 ml/kg, respectively, and a BH:LH ratio of 0.756 +/- 0.029. The latter value may have reflected an overestimation of plasma volume by the 125I-labeled albumin procedure.