Selective accumulation of delphinidin derivatives in tobacco using a putative flavonoid 3′,5′-hydroxylase cDNA from Campanula medium
Selective accumulation of delphinidin derivatives in tobacco using a putative flavonoid 3′,5′-hydroxylase cDNA from Campanula medium
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DOI：
10.1271/bbb.67.161
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发表时间：
2003-01-01
影响因子：
1.6
通讯作者：
Kikuchi, Y
中科院分区：
文献类型：
--
作者：
Okinaka, Y;Shimada, Y;Kikuchi, Y
蓝色花通常含有3 '，5'-羟基化的花青素（飞燕草素衍生物）作为色素，其仅在类黄酮3 '，5'-羟化酶（F3 '5'H）存在下形成。因此，F3 '5'H基因的异源表达为许多缺少蓝色开花品种的观赏植物提供了产生新的蓝色花的机会。然而，我们以前的研究表明，在表达F3 '5'H的植物中获得良好的飞燕草素衍生物积累是困难的。本文报道了从坎特伯雷风铃草（Campanula medium）中分离的一个推定的F3 '5'H cDNA（Ka 1）及其在烟草中的表达。令人惊讶的是，与其他F3 '5'H cDNA相比，Kal编码具有独特一级结构的蛋白质，该蛋白质在飞燕草素衍生物（高达总花青素的99%）的积累中赋予高能力，并产生新的紫色花。这些结果表明，在F3 '5'H cDNA中，Ka 1是通过基因工程创造优良蓝色花的最佳遗传资源。
Blue flowers generally contain 3',5'-hydroxylated anthocyanins (delphinidin derivatives) as pigments, which are formed only in the presence of flavonoid 3',5'hydroxylases (F3'5'H). Heterologous expression of a F3'5'H gene therefore provides an opportunity to produce novel blue flowers for a number of ornamental plants missing blue flowering varieties. However, our previous study indicated difficulties in obtaining good accumulation of delphinidin derivatives in plants expressing F3'5'H. Here we report the isolation of a putative F3'5'H cDNA (Ka1) from canterbury bells (Campanula medium) and its expression in tobacco. Surprisingly, compared with other F3'5'H cDNAs, Kal encoded a protein with a unique primary structure that conferred high competence in the accumulation of delphinidin derivatives (up to 99% of total anthocyanins) and produced novel purple flowers. These results suggest that, among F3'5'H cDNAs, Ka1 is the best genetic resource for the creation of fine blue flowers by genetic engineering.