The Involvement of Ferredoxin-NADP Reductase in the Photosynthetic Cyclic Electron Transport.
The Involvement of Ferredoxin-NADP Reductase in the Photosynthetic Cyclic Electron Transport.
复制标题
铁氧还蛋白-NADP 还原酶参与光合循环电子传输。
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
Ying
中科院分区：
文献类型：
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作者：
Ji;Ying
肝素作为铁氧化还蛋白-NADP还原酶（FNR）的抑制剂，在极低浓度（微摩尔）下强烈抑制Fd介导的菠菜类囊体中NADP的光还原，并抑制Fd/NADP诱导的延迟发光。在厌氧条件下，当DCMU存在阻止光系统II的电子转移时，可以测量fd介导的光系统I周围的循环电子传递，如9-氨基吖啶的猝灭。肝素可以强烈抑制这一循环，但对pms介导的光系统I周围的循环电子传递没有显著影响，肝素没有抑制光系统I到铁氧还蛋白的电子传递，这一点通过测量铁氧还蛋白的光还原得到了证明。上述结果表明，肝素通过抑制FNR活性抑制铁氧化还原素介导的循环电子传递，支持FNR参与循环电子传递的假说。
As an inhibitor of ferredoxin-NADP reductase (FNR), heparin strongly inhibited the Fd-mediated photoreduction of NADP in spinach thylakoids at very low concentration (micromole), and also inhibited the delayed light emission induced by Fd/NADP. Under anaerobic conditions, when DCMU was present to block electron transfer from photosystem II, the Fd-mediated cyclic electron transport around photosystem I as indicated by the quenching of 9-aminoacridine can be measured. Heparin could strongly inhibit this cycle, but had no significant effect on the PMS-mediated cyclic electron transport around photosystem I. Heparin did not inhibit the electron transport from phototsystem I to ferredoxin, which was demonstrated by measuring the photoreduction of ferredoxin. The above results indicate that heparin inhibits ferredoxin-mediated cyclic electron transport by inhibiting the activity of FNR, and support the hypothesis of the involvement of FNR in cyclic electron transport.