A hand-held surface plasmon resonance biosensor for the detection of ricin and other biological agents
A hand-held surface plasmon resonance biosensor for the detection of ricin and other biological agents
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DOI：
10.1016/j.bios.2007.11.005
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发表时间：
2008-02-28
影响因子：
12.6
通讯作者：
Davis, T. J.
中科院分区：
文献类型：
--
作者：
Feltis, B. N.;Sexton, B. A.;Davis, T. J.
对可现场部署的生物传感器设备的需求持续存在。我们已经构建了一个完全独立的手持生物传感器，基于表面等离子体共振技术。传感器单元的尺寸为15 x 8cm，重量为600g，由9v电池供电。我们使用校准的蔗糖溶液表征了传感器的响应性，并且能够测量小至3.3 x 10（-6）折射率单位的变化。为了证明传感器的功能，我们制备了带有生物毒素蓖麻毒素特异性抗体片段的表面。我们能够在10分钟内检测到200 ng/mL的蓖麻毒素，这大约是最小致死剂量的2500倍。我们还能够在第二个10分钟窗口内验证阳性结合。该传感器展示了开发完全集成的手持传感器设备所需的重要步骤，并将构成在不久的将来开发的多分析物系统的基础。它也代表了第一个完全手持的SPR设备，不需要外部电源或计算机连接来操作。(c) 2007 Elsevier B.V.版权所有
There is an ongoing need for field-deployable biosensor devices. We have constructed a fully self-contained, hand-held biosensor, based on the surface plasmon resonance technique. The dimensions of the sensor unit are 15 x 8 cm, the weight is 600 g and it is powered by a 9 V battery. We have characterised the responsiveness of the sensor using calibrated sucrose solutions and were able to measure changes as small as 3.3 x 10(-6) refractive index units. To demonstrate functionality of the sensor, we have prepared surfaces with an antibody fragment specific for the biological toxin ricin. We were able to detect ricin at 200 ng/mL in 10 min, which is approximately 2500 times less than the minimum lethal dose. We were also able to verify positive binding within a second 10 min window. This sensor demonstrates important steps required for the development of fully integrated, hand-held sensor devices and will form the basis of a multi-analyte system, to be developed in the near future. It also represents the first completely hand-held SPR device, not requiring external power or a computer connection to operate. (c) 2007 Elsevier B.V. All rights reserved.